Some content of this application is unavailable at the moment.
If this situation persist, please contact us atFeedback&Contact
1. (WO2017038926) ANTIBODY SUBJECTED TO FLUORESCENT LABELING USING NUCLEOTIDE BINDING SITE (NBS) OF ANTIBODY
Latest bibliographic data on file with the International Bureau    Submit observation

Pub. No.: WO/2017/038926 International Application No.: PCT/JP2016/075620
Publication Date: 09.03.2017 International Filing Date: 01.09.2016
IPC:
G01N 33/533 (2006.01) ,G01N 21/64 (2006.01) ,C07K 16/18 (2006.01)
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33
Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48
Biological material, e.g. blood, urine; Haemocytometers
50
Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53
Immunoassay; Biospecific binding assay; Materials therefor
531
Production of immunochemical test materials
532
Production of labelled immunochemicals
533
with fluorescent label
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
21
Investigating or analysing materials by the use of optical means, i.e. using infra-red, visible, or ultra-violet light
62
Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
63
optically excited
64
Fluorescence; Phosphorescence
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
K
PEPTIDES
16
Immunoglobulins, e.g. monoclonal or polyclonal antibodies
18
against material from animals or humans
Applicants:
国立大学法人東京工業大学 TOKYO INSTITUTE OF TECHNOLOGY [JP/JP]; 東京都目黒区大岡山2丁目12番1号 2-12-1, Ookayama, Meguro-ku, Tokyo 1528550, JP
ウシオ電機株式会社 USHIO DENKI KABUSHIKI KAISHA [JP/JP]; 東京都千代田区丸の内一丁目6番5号 1-6-5, Marunouchi, Chiyoda-ku, Tokyo 1008150, JP
Inventors:
上田 宏 UEDA Hiroshi; JP
鄭 熙陳 JEONG Hee-Jin; JP
董 金華 DONG Jinhua; JP
阿部 亮二 ABE Ryoji; JP
Agent:
平木 祐輔 HIRAKI Yusuke; JP
藤田 節 FUJITA Takashi; JP
田中 夏夫 TANAKA Natsuo; JP
Priority Data:
2015-17407503.09.2015JP
Title (EN) ANTIBODY SUBJECTED TO FLUORESCENT LABELING USING NUCLEOTIDE BINDING SITE (NBS) OF ANTIBODY
(FR) ANTICORPS SOUMIS À UN MARQUAGE FLUORESCENT AU MOYEN D’UN SITE DE LIAISON DE NUCLÉOTIDE (NBS) D’ANTICORPS
(JA) 抗体のヌクレオチド結合部位(NBS)を利用して蛍光標識された抗体
Abstract:
(EN) Provided are: a method for using a nucleotide binding site present in the variable domain of the antibody to prepare, through a photochemical modification method, a fluorescent labeled antibody in which the fluorescence intensity changes with the binding of an antigen; and a fluorescent labeled antibody obtained thereby. A labeled antibody for fluoroimmunoassay in which a compound for labeling a nucleotide binding site (NBS) of the antibody and having indole butyric acid (IBA) and a fluorescent group bound thereto is conjugated to a nucleotide binding site in the antibody. The compound is represented by formula (1) (in the formula, F is a fluorescent group, and R is a linking group selected from the group consisting of an alkyl group, alkenyl group, alkynyl group, C4–C10 cycloalkyl group, C4–C10 heterocyclyl group, C4–C10 aryl group, C4–C10 heteroaryl group, arylene group, ether group, ester group, PEG group, sulfide group, amide group, ketone group, and sulfamide group, the groups being substituted or unsubstituted, and combinations thereof).
(FR) La présente invention concerne : un procédé d’utilisation d’un site de liaison de nucléotide présent dans le domaine variable de l’anticorps pour préparer, par l’intermédiaire d’un procédé de modification photochimique, un anticorps marqué fluorescent dans lequel l’intensité de fluorescence change avec la liaison d’un antigène ; et un anticorps marqué fluorescent obtenu ainsi. Un anticorps marqué pour fluoro-immunodosage dans lequel un composé pour marquer un site de liaison nucléotidique (NBS) de l’anticorps et comportant de l’acide indolebutyrique (IBA) et un groupe fluorescent lié à celui-ci est conjugué à un site de liaison de nucléotide dans l’anticorps. Le composé est représenté par la formule (1) (dans la formule, F est un groupe fluorescent, et R est un groupe de liaison choisi dans le groupe constitué d’un groupe alkyle, un groupe alcényle, un groupe alcynyle, un groupe cycloalkyle en C4-C10, un groupe hétérocyclyle en C4-C10, un groupe aryle en C4-C10, un groupe hétéroaryle en C4-C10, un groupe arylène, un groupe éther, un groupe ester, un groupe PEG, un groupe sulfure, un groupe amide, un groupe cétone et un groupe sulfamide, les groupes étant substitués ou non substitués, et des combinaisons de ceux-ci).
(JA) 抗体の可変領域内部に存在するヌクレオチド結合部位を利用して、光化学修飾法により、抗原の結合により蛍光強度が変化する蛍光標識抗体を作製する方法、及び得られた蛍光標識抗体の提供。 下記式(1):(式中、Fは蛍光基であり、Rは、置換又は未置換の、アルキル基、アルケニル基、アルキニル基、C4~C10シクロアルキル基、C4~C10ヘテロシクリル基、C4~C10アリール基、C4~C10ヘテロアリール基、アリーレン基、エーテル基、エステル基、PEG基、スルフィド基、アミド基、ケトン基、スルファミド基及びそれらの組合せからなる群より選択されるリンカー基である)で表される、インドール酪酸(IBA)と蛍光基が結合している、抗体のヌクレオチド結合部位(NBS)標識用化合物が抗体内のヌクレオチド結合部位にコンジュゲートされている蛍光免疫測定用標識抗体。
front page image
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, KE, KG, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: Japanese (JA)
Filing Language: Japanese (JA)