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1. (WO2017038835) METHOD OF PRODUCING PLASTID TRANSFORMANTS

Pub. No.:    WO/2017/038835    International Application No.:    PCT/JP2016/075375
Publication Date: Fri Mar 10 00:59:59 CET 2017 International Filing Date: Thu Aug 25 01:59:59 CEST 2016
IPC: C12N 15/82
Applicants: HONDA MOTOR CO., LTD.
Inventors: YAMAGUCHI, Asuka
OKUMA, Jiro
KONDO, Yasuhiro
HIROSE, Yoshitsugu
Title: METHOD OF PRODUCING PLASTID TRANSFORMANTS
Abstract:
A method of producing plastid transformants including a process (a) in which genes coding for proteins having a function of neutralizing nitrite toxicity are introduced into plant tissues and a process (b) in which the plant tissues into which the genes are inserted in the process (a) are cultured in a medium containing nitrite nitrogen having a concentration at which growth of wild type plant cells is suppressed or a concentration at which regeneration of wild type plants is suppressed and thus plastid transformants obtained by inserting the genes into plastid genomes are screened.