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1. (WO2017038064) DROPLET MICROFLUIDIC DEVICE AND METHODS OF SENSING THE RESULT OF AN ASSAY THEREIN

Pub. No.:    WO/2017/038064    International Application No.:    PCT/JP2016/003882
Publication Date: Fri Mar 10 00:59:59 CET 2017 International Filing Date: Sat Aug 27 01:59:59 CEST 2016
IPC: G01N 33/579
Applicants: SHARP LIFE SCIENCE (EU) LIMITED
ASSOCIATES OF CAPE COD INCORPORATED
Inventors: HADWEN, Benjamin James
JACOBS, Adrian Marc Simon
HECTOR, Jason Roderick
BROWNLOW, Michael James
ADACHI, Masahiro
SKINNER, Alison Mary
CHILDS, Mark
Title: DROPLET MICROFLUIDIC DEVICE AND METHODS OF SENSING THE RESULT OF AN ASSAY THEREIN
Abstract:
A method of determining the result of an assay in a microfluidic device includes the steps of: dispensing a sample droplet onto a first portion of an electrode array of the microfluidic device; dispensing a reagent droplet onto a second portion of the electrode array of the microfluidic device; controlling actuation voltages applied to the electrode array to mix the sample droplet and the reagent droplet into a product droplet; sensing a dynamic property of the product droplet; and determining an assay of the sample droplet based on the sensed dynamic property. The dynamic property is a physical property of the product droplet that influences a transport property of the product droplet on the electrode array. Example dynamic properties of the product droplet include the moveable state, split-able state, and viscosity based on droplet properties. The method may be used to perform an amoebocyte lysate (LAL) assay.