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1. (WO2017037875) METHOD FOR ACQUIRING 3-DIMENSIONAL IMAGE, METHOD FOR DISPLAYING 3-DIMENSIONAL IMAGE, AND DEVICE FOR ACQUIRING 3-DIMENSIONAL IMAGE

Pub. No.:    WO/2017/037875    International Application No.:    PCT/JP2015/074867
Publication Date: Fri Mar 10 00:59:59 CET 2017 International Filing Date: Wed Sep 02 01:59:59 CEST 2015
IPC: C12M 1/00
C12Q 1/66
G01N 21/76
G06T 1/00
Applicants: OLYMPUS CORPORATION
オリンパス株式会社
Inventors: AKIYOSHI, Ryutaro
秋吉 竜太郎
Title: METHOD FOR ACQUIRING 3-DIMENSIONAL IMAGE, METHOD FOR DISPLAYING 3-DIMENSIONAL IMAGE, AND DEVICE FOR ACQUIRING 3-DIMENSIONAL IMAGE
Abstract:
A method for acquiring a 3-dimensional image, for acquiring an image of faint light emitted from a biological sample and constructing a 3-dimensional image, includes the steps of (S1) performing first image acquisition for acquiring a 2-dimensional image, (S2) determining an exposure time on the basis of the first image acquisition, (S3) acquiring the thickness of the biological sample, (S4) acquiring an allowed time in which second image acquisition for acquiring a plurality of 2-dimensional images while changing a focal position in an optical axis direction is allowed, (S5) determining a step width in the second image acquisition on the basis of the exposure time, the thickness of the biological sample, and the allowed time, (S6) performing second image acquisition on the basis of the exposure time and the step width, and (S7) constructing a 3-dimensional image on the basis of the obtained plurality of 2-dimensional images.