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1. (WO2017037664) DEVICE AND METHOD FOR DETECTING BIOLOGICAL CONTAMINANTS, IN PARTICULAR COLIFORM BACTERIA IN WATER SAMPLES
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Pub. No.: WO/2017/037664 International Application No.: PCT/IB2016/055263
Publication Date: 09.03.2017 International Filing Date: 02.09.2016
IPC:
C12Q 1/04 (2006.01) ,C12Q 1/10 (2006.01) ,G01N 21/03 (2006.01) ,G01N 33/18 (2006.01) ,G01N 21/64 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
02
involving viable micro-organisms
04
Determining presence or kind of micro-organism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
02
involving viable micro-organisms
04
Determining presence or kind of micro-organism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
10
Enterobacteria
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
21
Investigating or analysing materials by the use of optical means, i.e. using infra-red, visible, or ultra-violet light
01
Arrangements or apparatus for facilitating the optical investigation
03
Cuvette constructions
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33
Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
18
Water
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
21
Investigating or analysing materials by the use of optical means, i.e. using infra-red, visible, or ultra-violet light
62
Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
63
optically excited
64
Fluorescence; Phosphorescence
Applicants:
BIOSENSING TECHNOLOGIES S.R.L. [IT/IT]; Via Ausonia, 20 I-00171 Roma, IT
Inventors:
PEZZOTTI ESCOBAR, Gianni; IT
BUONASERA, Katia; IT
PEZZOTTI ESCOBAR, Ittalo Francisco; IT
CANO QUINTERO, Juan Bernardo; CO
Agent:
SARPI, Maurizio; IT
FEZZARDI, Antonio; IT
Priority Data:
10201500004784502.09.2015IT
Title (EN) DEVICE AND METHOD FOR DETECTING BIOLOGICAL CONTAMINANTS, IN PARTICULAR COLIFORM BACTERIA IN WATER SAMPLES
(FR) DISPOSITIF ET PROCÉDÉ DE DÉTECTION DE CONTAMINANTS BIOLOGIQUES, EN PARTICULIER DES BACTÉRIES COLIFORMES DANS DES ÉCHANTILLONS D’EAU
Abstract:
(EN) Described herein is a thermostatted device, as well as the corresponding method, for detecting biological contaminants, in particular coliform bacteria in a water sample, which are designed for making measurements of luminescence at different wavelengths, by resorting to a flow cell equipped with a plurality of measurement chambers, hydraulically connected but not in optical communication with one another, into which the water sample is conveyed without the use of cuvettes, there being provided in each of said chambers means for exciting the sample present and detecting the luminescence of the bacterium, an inlet for injection of the fluorophore, and microvalves and capillary vessels for supplying to the individual measurement chambers said fluorophore in the amount necessary for making the measurement.
(FR) La présente invention concerne un dispositif thermostaté, ainsi que le procédé correspondant, pour détecter des contaminants biologiques, en particulier des bactéries coliformes dans un échantillon d’eau, qui sont conçus pour effectuer des mesures de luminescence à différentes longueurs d’onde, au moyen d’une cellule à circulation équipée d’une pluralité de chambres de mesure, hydrauliquement raccordées mais pas en communication optique les unes avec les autres, dans laquelle l’échantillon d’eau est transporté sans l’utilisation de cuvettes, chacune desdites chambres étant pourvue de moyens pour exciter l’échantillon présent et détecter la luminescence de la bactérie, une entrée pour l’injection du fluorophore, et des microvalves et des vaisseaux capillaires pour distribuer dans les chambres de mesure individuelles ledit fluorophore dans la quantité nécessaire pour effectuer la mesure.
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Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: Italian (IT)