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1. (WO2017037656) METHOD FOR BIDIRECTIONAL SEQUENCING
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Pub. No.: WO/2017/037656 International Application No.: PCT/IB2016/055251
Publication Date: 09.03.2017 International Filing Date: 01.09.2016
IPC:
C12Q 1/68 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
68
involving nucleic acids
Applicants:
OSBORNE, Robert [GB/GB]; GB
Inventors:
OSBORNE, Robert; GB
Priority Data:
1515557.502.09.2015GB
Title (EN) METHOD FOR BIDIRECTIONAL SEQUENCING
(FR) MÉTHODE DE SÉQUENÇAGE BIDIRECTIONNEL
Abstract:
(EN) Described herein is a method of sequencing, comprising: splitting an asymmetrically tagged library into a plurality of subsamples, tagging the adaptor-ligated DNA in the sub- samples with sequence tags that identify the subsamples, optionally pooling the sub-samples, sequencing polynucleotides from each of the tagged sub-samples, or copies of the same, to produce sequence reads each comprising: i. a sub-sample identifier sequence and ii. the sequence of at least part of a fragment in the sample, wherein some of the sequence reads are derived from the top strand of a fragment in the sample and some of the sequence reads of are derived from the bottom strand of the same fragment.
(FR) L'invention concerne un procédé de séquençage, consistant à : diviser une banque marquée de manière asymétrique en une pluralité de sous-échantillons, marquer l'ADN ligaturé à des adaptateurs dans les sous-échantillons avec des étiquettes de séquence qui identifient les sous-échantillons, rassembler optionnellement les sous-échantillons, séquencer des polynucléotides issus de chacun des sous-échantillons marqués, ou des copies de ceux-ci, pour produire des lectures de séquence comprenant chacune : i. une séquence identifiant le sous-échantillon et ii. la séquence d'au moins une partie d'un fragment dans l'échantillon, une partie des lectures de séquence étant dérivée du brin supérieur d'un fragment dans l'échantillon et une partie des lectures de séquence étant dérivée du brin inférieur du même fragment.
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Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)