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1. (WO2017036916) PROCESS TO MANUFACTURE CROSS-LINKED ENZYME AGGREGATES

Pub. No.:    WO/2017/036916    International Application No.:    PCT/EP2016/070093
Publication Date: Fri Mar 10 00:59:59 CET 2017 International Filing Date: Fri Aug 26 01:59:59 CEST 2016
IPC: C12N 9/96
C11D 3/386
Applicants: UNILEVER PLC
UNILEVER N.V.
CONOPCO, INC., D/B/A UNILEVER
Inventors: DE ROSE, Simone, Antonio
DOWD, Andrew
LANG, Dietmar, Andreas
LITTLECHILD-BOND, Jennifer, Ann
NOVAK, Halina, Rose
PARRY, Neil, James
SINGH, Sukriti
Title: PROCESS TO MANUFACTURE CROSS-LINKED ENZYME AGGREGATES
Abstract:
A process for the manufacture of cross-linked enzyme aggregates comprising the steps of: 1) providing a liquid mixture of the enzyme; 2) precipitating the enzyme comprised by the liquid mixture; 3) cross-linking the enzyme by addition of a cross-linking agent, having at least two reactive groups suitable for cross-linking amino acids; 4) stopping the cross-linking by addition of a suitable buffer; wherein step 2) is performed in the presence of a compound selected from a crown ether or a surfactant; and wherein step 4) is preferably performed when the mean diameter of the particles is at most 4.0 µm, as measured using a Turbiscan MA200 at 21 degrees Celsius, using the Stokes-Einstein equation to measure the particle size.