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1. (WO2017036495) METHOD OF FERMENTATIVE ALPHA-IONONE PRODUCTION

Pub. No.:    WO/2017/036495    International Application No.:    PCT/EP2015/069751
Publication Date: Fri Mar 10 00:59:59 CET 2017 International Filing Date: Sat Aug 29 01:59:59 CEST 2015
IPC: C12P 23/00
C12N 15/63
C12P 5/00
Applicants: PHYTOWELT GREENTECHNOLOGIES GMBH
Inventors: JACH, Guido
AZDOUFFAL, Sanae
SCHULLEHNER, Katrin
WELTERS, Peter
NATANEK, Angela
Title: METHOD OF FERMENTATIVE ALPHA-IONONE PRODUCTION
Abstract:
The present invention relates to a method of preparing enantiomerically pure alpha-ionone. The invention further relates to a nucleic acid comprising a sequence that encodes a lycopene epsilon-cyclase (EC), a lycopene epsilon-cyclase (EC), plasmids that encode components of alpha-ionone biosynthesis and a microorganism containing heterologous nucleotide sequences that encode the enzymes geranylgeranyl diphosphate synthase, isopentenyl diphosphate isomerase (IPI), phytoene desaturase/dehydrogenase (crtl), phytoene synthase (crt B) and lycopene epsilon-cyclase (EC) or geranylgeranyl diphosphate synthase, isopentenyl diphosphate isomerase (IPI), phytoene desaturase/dehydrogenase (crtl), phytoene synthase (crt B), lycopene epsilon-cyclase (EC) and carotenoid cleavage dioxygenase (CCD1). The invention further relates to a method of producing high-purity epsilon-carotene.