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1. (WO2017036374) METHOD FOR AMPLIFYING DNA

Pub. No.:    WO/2017/036374    International Application No.:    PCT/CN2016/097208
Publication Date: Fri Mar 10 00:59:59 CET 2017 International Filing Date: Tue Aug 30 01:59:59 CEST 2016
IPC: C12N 15/10
Applicants: SHANGHAI XUKANG MEDICAL SCIENCE & TECHNOLOGY CO., LTD
上海序康医疗科技有限公司
Inventors: LU, Sijia
陆思嘉
YIN, Guangjun
殷广军
Title: METHOD FOR AMPLIFYING DNA
Abstract:
Provided is a method for amplifying the genomic DNA of a cell, wherein the method comprises: (a) providing a reaction mixture, wherein the reaction mixture comprises the genomic DNA, a first class of primer, a second class of primer, a mixture of nucleotide monomers and a nucleic acid polymerase, wherein the first class of primer comprises a universal sequence and a variable sequence from the 5' terminus to the 3' terminus, wherein the universal sequence consists of three or two of four bases G, A, C and T, with the proviso that the universal sequence does not comprise G and C at the same time, and the second class of primer comprises the universal sequence and does not comprise the variable sequence; (b) placing the reaction mixture in a first temperature cycle program, such that the variable sequence of the first class of primer is capable of matching the genomic DNA and amplifying the genomic DNA to obtain a genomic amplification product, wherein the 5' terminus of the genomic amplification product comprises the universal sequence and the 3' terminus comprises a complementary sequence of the universal sequence; (c) placing the reaction mixture obtained in step (b) into a second temperature cycle program, such that the universal sequence of the second class of primer is capable of matching the 3' end of the genomic amplification product and amplifying the genomic amplification product to obtain an expanded genomic amplification product, wherein the reaction mixture is provided prior to step (b) and step (c). Also provided is a kit for amplifying the genomic DNA.