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1. (WO2017036143) GROUP A MENINGOCOCCAL CAPSULAR POLYSACCHARIDE CONJUGATE VACCINE AND PREPARATION METHOD THEREOF

Pub. No.:    WO/2017/036143    International Application No.:    PCT/CN2016/078090
Publication Date: Fri Mar 10 00:59:59 CET 2017 International Filing Date: Fri Apr 01 01:59:59 CEST 2016
IPC: A61K 39/385
A61K 39/095
A61P 31/04
Applicants: CHENGDU OLYMVAX BIOPHARMACEUTICAL CO.,LTD
成都欧林生物科技股份有限公司
Inventors: WU, Changhua
伍长华
WANG, Qian
王乾
LI, Hongguang
李洪光
Title: GROUP A MENINGOCOCCAL CAPSULAR POLYSACCHARIDE CONJUGATE VACCINE AND PREPARATION METHOD THEREOF
Abstract:
A group A meningococcal capsular polysaccharide conjugate vaccine comprises a conjugate of group A meningococcal capsular polysaccharide and tetanus toxoid. A preparation method thereof comprises: diluting and activating group A meningococcal capsular polysaccharide, and coupling the same with sebacic dihydrazid to obtain a derivative of group A meningococcal polysaccharide and sebacic dihydrazid; and reacting, using carbodiimide as a catalyst, the derivative of group A meningococcal polysaccharide and sebacic dihydrazid with a tetanus toxoid solution, to obtain a conjugate of group A meningococcal capsular polysaccharide and a carrier protein. The present invention uses sebacic dihydrazide as a coupling agent during the preparation process of the group A meningococcal conjugate vaccine; compared with adipic dihydrazide, sebacic dihydrazide has two additional methylene groups on the carbon chain and a longer molecular chain, and accordingly better overcomes steric hindrance between large biomolecules, thereby improving the conjugate yield.