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1. (WO2017036140) A-GROUP NEISSERIA MENINGITIDIS CULTIVATION METHOD

Pub. No.:    WO/2017/036140    International Application No.:    PCT/CN2016/078086
Publication Date: Fri Mar 10 00:59:59 CET 2017 International Filing Date: Fri Apr 01 01:59:59 CEST 2016
IPC: C12N 1/20
C12R 1/36
Applicants: CHENGDU OLYMVAX BIOPHARMACEUTICAL CO., LTD
成都欧林生物科技股份有限公司
Inventors: ZHU, Chong
朱冲
MI, Qiang
米强
CHEN, Daoyuan
陈道远
Title: A-GROUP NEISSERIA MENINGITIDIS CULTIVATION METHOD
Abstract:
An A-group Neisseria meningitidis cultivation method comprises the following steps: (1) preparing purified strains of a working seed lot; (2) opening the strains of the working seed lot, inoculating same into a semi-synthetic culture medium, performing cultivation at the temperature of 35-37°C for 16-24 hours, and then preparing an appropriate quantity of seeds for production after three-generation multiplication culture; and (3) inoculating the seeds for production into a fermentation tank, and performing cultivation for 6-12 hours, the culture medium in the fermentation tank being a semi-synthetic culture medium, the temperature being 35-37°C, the pH of the culture medium being 7.0-7.2, the stirring speed in the fermentation tank being 140-150 r/min, and the inoculation quantity being 100,000 ml to 150,000 ml.