Search International and National Patent Collections

1. (WO2017036137) METHOD FOR PREPARING POLYSACCHARIDE-ADH DERIVATIVE BY MEANS OF A-GROUP NEISSERIA MENINGITIDIS REFINED POLYSACCHARIDE

Pub. No.:    WO/2017/036137    International Application No.:    PCT/CN2016/078083
Publication Date: Fri Mar 10 00:59:59 CET 2017 International Filing Date: Fri Apr 01 01:59:59 CEST 2016
IPC: A61K 39/095
A61P 31/04
Applicants: CHENGDU OLYMVAX BIOPHARMACEUTICAL CO., LTD
成都欧林生物科技股份有限公司
Inventors: WU, Qiang
吴强
WU, Changhua
伍长华
TAN, Yong
谭勇
Title: METHOD FOR PREPARING POLYSACCHARIDE-ADH DERIVATIVE BY MEANS OF A-GROUP NEISSERIA MENINGITIDIS REFINED POLYSACCHARIDE
Abstract:
Disclosed is a method for preparing a polysaccharide-ADH derivative by means of A-group Neisseria meningitidis refined polysaccharide, comprising the following steps: (1) mixing Neisseria meningitidis refined polysaccharide and water for injection to prepare a polysaccharide solution, and adjusting pH to be 9.0; (2) dissolving CDAP into a CDAP solution by using acetonitrile, adding the CDAP solution to the polysaccharide solution according to a weight proportion: polysaccharide:CDAP=1:0.4-0.6, and performing stirring for 2-5 minutes at room temperature to prepare a CDAP mixed liquid; (3) dissolving ADH into an ADH solution with the concentration of 100 mg/ml by using NaHCO3 with the concentration of 0.2 mol/L, adding the ADH solution to the CDAP mixed liquid according to a weight proportion: polysaccharide:ADH=1:3-5, and performing stirring for an hour at room temperature; and (4) finally, performing filtering and freeze drying by using an ultra-filtration membrane module.