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1. (WO2017035347) SCREENING OF NUCLEIC ACID SEQUENCE VARIATIONS VIA RANDOM LOADING OF OPTICALLY-ENCODED PARTICLES
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Pub. No.: WO/2017/035347 International Application No.: PCT/US2016/048687
Publication Date: 02.03.2017 International Filing Date: 25.08.2016
IPC:
C12P 19/34 (2006.01) ,C12Q 1/68 (2006.01) ,G01N 21/64 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
P
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
19
Preparation of compounds containing saccharide radicals
26
Preparation of nitrogen-containing carbohydrates
28
N-glycosides
30
Nucleotides
34
Polynucleotides, e.g. nucleic acids, oligoribonucleotides
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
68
involving nucleic acids
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
21
Investigating or analysing materials by the use of optical means, i.e. using infra-red, visible, or ultra-violet light
62
Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
63
optically excited
64
Fluorescence; Phosphorescence
Applicants:
SAZER, Daniel [US/US]; US
THE BROAD INSTITUTE, INC. [US/US]; 415 Main Street Cambridge, MA 02142, US
MASSACHUSETTS INSTITUTE OF TECHNOLOGY [US/US]; 77 Massachusetts Avenue Cambridge, MA 02139, US
Inventors:
SAZER, Daniel; US
FELDMAN, David; US
BLAINEY, Paul; US
Agent:
NIX, F., Brent; US
Priority Data:
62/209,70925.08.2015US
Title (EN) SCREENING OF NUCLEIC ACID SEQUENCE VARIATIONS VIA RANDOM LOADING OF OPTICALLY-ENCODED PARTICLES
(FR) CRIBLAGE DE VARIATIONS DE SÉQUENCE D’ACIDES NUCLÉIQUES PAR L'INTERMÉDIAIRE DE LA CHARGE ALÉATOIRE DE PARTICULES OPTIQUEMENT CODÉES
Abstract:
(EN) Embodiments disclosed herein provide reagents and methods for high-throughput screening of nucleic acid sequence variations in nucleic acid containing specimens. Nucleic acid specimens to be screened are loaded into separate discrete volumes. Optically encoded particles are used to deliver primers to amplify one or more sequences comprising the nucleic acid sequence variation. The optically encoded particles may be delivered to the discrete volumes randomly resulting in a random combination of optically encoded particles in each well, or a unique combination of optically encoded particles may be specifically assigned to each discrete volume. The observable combination of optically encoded particles may then be used to identify each discrete volume.
(FR) Les modes de réalisation de la présente invention décrivent des réactifs et des procédés de criblage à haut débit de variations de séquence d’acides nucléiques dans des échantillons contenant un acide nucléique. Les échantillons d’acide nucléique à cribler sont chargés en volumes discrets séparés. Les particules optiquement codées sont utilisées pour distribuer des amorces afin d’amplifier une ou plusieurs séquences comprenant la variation de séquence d’acides nucléiques. Les particules optiquement codées peuvent être distribuées aux volumes discrets résultants de manière aléatoire en une combinaison aléatoire de particules optiquement codées dans chaque puits, ou une combinaison unique de particules optiquement codées peut être spécifiquement attribuée à chaque volume discret. La combinaison observable des particules optiquement codées peut ensuite être utilisée pour identifier chaque volume discret.
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Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)