Search International and National Patent Collections

1. (WO2017035191) SYSTEMS AND METHODS FOR ENRICHING A BACTERIAL STRAIN FROM A TARGET BACTERIAL SYSTEM

Pub. No.:    WO/2017/035191    International Application No.:    PCT/US2016/048317
Publication Date: Fri Mar 03 00:59:59 CET 2017 International Filing Date: Thu Aug 25 01:59:59 CEST 2016
IPC: C12N 1/20
A61K 35/38
A61K 35/37
A61K 35/74
C12R 1/01
Applicants: NUBYIOTA LLC
Inventors: ALLEN-VERCOE, Emma
Title: SYSTEMS AND METHODS FOR ENRICHING A BACTERIAL STRAIN FROM A TARGET BACTERIAL SYSTEM
Abstract:
The present invention is a method of enriching at least one bacterial species from a target bacterial system, including: culturing the target bacterial ecosystem in a culture media in a single-stage chemostat under the following conditions: (i) a system retention time of about 5 to about 290 hours, (ii) a temperature of about 37°C, (iii) a pH of about 6.8 to 7, and (iv) maintenance of anaerobic conditions to the chemostat for a time sufficient to enrich the at least one bacterial species; where the culture media comprises a prepared starch substrate, and where the target bacterial system is a fecal derived sample obtained from a patient that has not been treated with an antibiotic for at least 6 months.