Search International and National Patent Collections

1. (WO2017034699) METHODS FOR INACTIVATION AND EXTRACTION OF ACID-FAST BACTERIA FROM LIQUID MEDIA FOR CHARACTERIZATION AND/OR IDENTIFICATION USING MASS SPECTROMETRY

Pub. No.:    WO/2017/034699    International Application No.:    PCT/US2016/042537
Publication Date: Fri Mar 03 00:59:59 CET 2017 International Filing Date: Sat Jul 16 01:59:59 CEST 2016
IPC: C12Q 1/24
C12M 1/00
C12N 1/02
Applicants: BIOMERIEUX, INC.
DEOL, Parampal
MILLER, Eric
MORENO, Erik
TOTTY, Heather
Inventors: DEOL, Parampal
MILLER, Eric
MORENO, Erik
TOTTY, Heather
Title: METHODS FOR INACTIVATION AND EXTRACTION OF ACID-FAST BACTERIA FROM LIQUID MEDIA FOR CHARACTERIZATION AND/OR IDENTIFICATION USING MASS SPECTROMETRY
Abstract:
The present invention is directed to a method and kit for inactivation of acid -fast bacteria. In some embodiments, the method may include: transferring a sample from a liquid culture containing acid-fast bacteria to a first tube, wherein the first tube comprises a body, a first end to the body having an opening, and a second end to the body having a frustoconical portion ending in a concave tip; centrifuging the tube to pellet the acid-fast bacteria in the concave tip and subsequently decanting a supernatant; resuspending the acid-fast bacteria pellet in alcohol; transferring the suspension to a second tube containing beads; agitating the second tube to disrupt acid-fast bacteria cells; and incubating the suspension to inactivate the acid-fast bacteria in the test sample. The method may also include identifying the acid-fast bacteria with mass spectrometry.