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1. (WO2017034387) A METHOD OF PRODUCING LI G NOCELL 11 LOL YT I C ENZYMES USING A SPECIES OF WHITE ROT FUNGI BY SOUP STATE FERMENTATION (SSF)

Pub. No.:    WO/2017/034387    International Application No.:    PCT/MY2016/000055
Publication Date: Fri Mar 03 00:59:59 CET 2017 International Filing Date: Sat Aug 27 01:59:59 CEST 2016
IPC: C12P 21/00
C12N 1/14
Applicants: MALAYSIAN PALM OIL BOARD
Inventors: IDRIS, Abu Seman
YUVARANI, Naidu Raju Naidu
AHMAD, Kushairi Din
NORMAN, Hj Kamarudin
Title: A METHOD OF PRODUCING LI G NOCELL 11 LOL YT I C ENZYMES USING A SPECIES OF WHITE ROT FUNGI BY SOUP STATE FERMENTATION (SSF)
Abstract:
The embodiments of the present invention relate to a method of producing lignocellulolytic enzymes using a species of white rot fungi by solid stale fermentation (SSF) is provided. The method comprises obtaining artificially modified fungus. The artificially modified fungus is Trammetes lactinea (GanoBF I ). An inoculum is prepared using said artificially modified fungus. A substrate is prepared using rubber-wood sawdust, vermiculite and rice bran. The said substrate is inoculated with the said prepared inoculum. The substrate is incubated to obtain the lignocellulolytic enzymes. The incubation is done tn a growth chamber at 25 ± 2°C under SSF static conditions. The fermentation is carried out of the said incubated substrate for at least 21 days.