Search International and National Patent Collections

1. (WO2017033898) CELL CULTURE DEVICE AND BIOLOGICAL SAMPLE PRODUCTION METHOD

Pub. No.:    WO/2017/033898    International Application No.:    PCT/JP2016/074404
Publication Date: Fri Mar 03 00:59:59 CET 2017 International Filing Date: Tue Aug 23 01:59:59 CEST 2016
IPC: C12M 3/00
C12M 1/00
C12M 1/42
C12N 5/07
Applicants: ASAHI GLASS COMPANY, LIMITED
旭硝子株式会社
Inventors: TABATA, Yasuhiko
田畑 泰彦
IDE, Masamichi
井手 正迪
KONISHI, Teppei
小西 哲平
KOGUCHI, Ryohei
小口 亮平
Title: CELL CULTURE DEVICE AND BIOLOGICAL SAMPLE PRODUCTION METHOD
Abstract:
The purpose of the present invention is to provide: a cell culture device which is capable of causing cells of various types to be adsorbed on a vessel surface during culture to promote organization thereof, is capable of desorbing a resultant biological sample without impairing the original function of the cells, and is capable of inhibiting desorption of the cells during culture; and a biological sample production method. This cell culture device 1 is provided with: a transparent culture vessel 10 in which a culture solution is accommodated; a dielectric layer 12 formed at the side of the inner bottom surface 10a of the culture vessel 10 such that a first surface 12a is in contact with the culture solution; a first electrode 14 provided to a second surface 12b of the dielectric layer 12 at the opposite side to the first surface 12a; a second electrode 16 provided inside the culture vessel 10 and away from the dielectric layer 12; and a voltage application mechanism 18 which applies a voltage between the first electrode 14 and the second electrode 16. The surface potential of the first surface 12a of the dielectric layer 12 changes as a result of applying the voltage between the first electrode 14 and the second electrode 16.