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1. (WO2017033809) CELL MEASUREMENT METHOD
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Pub. No.: WO/2017/033809 International Application No.: PCT/JP2016/073996
Publication Date: 02.03.2017 International Filing Date: 17.08.2016
IPC:
C12Q 1/06 (2006.01) ,G01N 21/27 (2006.01) ,G01N 33/48 (2006.01) ,G01N 33/483 (2006.01) ,C12M 1/00 (2006.01) ,C12N 5/09 (2010.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
02
involving viable micro-organisms
04
Determining presence or kind of micro-organism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
06
Quantitative determination
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
21
Investigating or analysing materials by the use of optical means, i.e. using infra-red, visible, or ultra-violet light
17
Systems in which incident light is modified in accordance with the properties of the material investigated
25
Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
27
using photo-electric detection
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33
Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48
Biological material, e.g. blood, urine; Haemocytometers
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33
Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48
Biological material, e.g. blood, urine; Haemocytometers
483
Physical analysis of biological material
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
M
APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
1
Apparatus for enzymology or microbiology
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5
Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
09
Tumour cells
Applicants:
倉敷紡績株式会社 KURASHIKI BOSEKI KABUSHIKI KAISHA [JP/JP]; 岡山県倉敷市本町7番1号 7-1, Honmachi, Kurashiki-shi, Okayama 7100054, JP
Inventors:
南條 祐子 NANJO, Yuko; JP
浅野 宏幸 ASANO, Hiroyuki; JP
宮川 功 MIYAGAWA, Isao; JP
高田 仁夫 TAKADA, Yoshio; JP
Agent:
河原 哲郎 KAWAHARA, Tetsuro; JP
Priority Data:
2015-16712226.08.2015JP
Title (EN) CELL MEASUREMENT METHOD
(FR) PROCÉDÉ DE MESURE DE CELLULES
(JA) 細胞測定方法
Abstract:
(EN) [Problem] To provide a highly accurate cell measurement method. [Solution] A cell measurement method that comprises: a step for staining cultured target cells with a dye; a step for obtaining a first image and a second image which are transmission images respectively to first light and second light, to said first light and second light, the aforesaid dye having different absorbances; a step for dividing both of the first image and the second image into a plurality of areas and then comparing the first image to the second image for each of the divided areas to thereby eliminate noise; and a step for integrating index values of cell amount in each of the divided area of the images from which the noise has been eliminated so as to evaluate the amount of the target cells.
(FR) Le problème décrit par la présente invention est de fournir un procédé de mesure de cellules de haute précision. La solution selon l'invention porte sur un procédé de mesure de cellules qui comprend : une étape consistant à colorer des cellules cibles cultivées avec un colorant; une étape consistant à obtenir une première image et une seconde image qui sont des images de transmission par rapport à une première lumière et à une seconde lumière respectivement, sous lesdites première lumière et seconde lumière, le colorant susmentionné présentant différents degrés d'absorbance; une étape consistant à diviser à la fois la première image et la seconde image en une pluralité de zones et à comparer ensuite la première image à la seconde image pour chacune des zones divisées pour ainsi éliminer le bruit; et une étape consistant à intégrer les valeurs d'indice de la quantité de cellules dans chacune des zones divisées des images à partir desquelles le bruit a été éliminé de manière à évaluer la quantité de cellules cibles.
(JA) [課題]精度の高い細胞測定方法を提供する。 [解決手段]培養された標的細胞を色素で染色する工程と、前記色素による吸光度が異なる第1の光および第2の光に対する透過像である第1画像および第2画像を取得する工程と、前記第1画像および前記第2画像のそれぞれを複数の分割領域に分割し、前記分割領域毎に前記第1画像と前記第2画像を比較してノイズを排除する工程と、前記ノイズを排除した画像において前記分割領域毎の細胞量の指標を積算することにより、前記標的細胞量を評価する工程とを有する細胞測定方法である。
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Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: Japanese (JA)
Filing Language: Japanese (JA)