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1. (WO2017033464) METHOD FOR PRODUCING PHYTOSPHINGOSINE OR SPHINGANINE
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Pub. No.: WO/2017/033464 International Application No.: PCT/JP2016/003866
Publication Date: 02.03.2017 International Filing Date: 24.08.2016
IPC:
C12N 9/12 (2006.01) ,C12P 13/00 (2006.01) ,C12N 1/19 (2006.01) ,C12R 1/865 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9
Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
10
Transferases (2.)
12
transferring phosphorus containing groups, e.g. kinases (2.7)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
P
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
13
Preparation of nitrogen-containing organic compounds
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
1
Micro-organisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving micro-organisms or compositions thereof; Processes of preparing or isolating a composition containing a micro-organism; Culture media therefor
14
Fungi; Culture media therefor
16
Yeasts; Culture media therefor
19
modified by introduction of foreign genetic material
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
R
INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C-C12Q75
1
Micro-organisms
645
Fungi
85
Saccharomyces
865
Saccharomyces cerevisiae
Applicants:
AJINOMOTO CO., INC. [JP/JP]; 15-1, Kyobashi 1-chome, Chuo-ku, Tokyo 1048315, JP
EVOLVA SA [CH/CH]; Duggingerstrasse 23, Reinach 4153, CH
Inventors:
SCHWAB, Markus; DE
BABAU, Maud; DK
FISCHER, David; CH
HATSCH, Anaelle; FR
TAVARES, Sabina de Andrade Pereira; CH
NIELSEN, Curt Aime Friis; CH
KLEIN, Jens; CH
WIRDNAM, Corina Daniela; CH
Agent:
KAWAGUCHI, Yoshiyuki; JP
SANUKI, Shinichi; JP
NIWA, Takeshi; JP
Priority Data:
2015-16469324.08.2015JP
62/208,93424.08.2015US
Title (EN) METHOD FOR PRODUCING PHYTOSPHINGOSINE OR SPHINGANINE
(FR) PROCÉDÉ DE PRODUCTION DE PHYTOSPHINGOSINE OU DE SPHINGANINE
Abstract:
(EN) A method for producing an objective substance such as phytosphingosine and sphinganine using yeast is provided. An objective substance is produced by cultivating yeast having an ability to produce the objective substance and modified so that the expression and/or activities of proteins encoded by LCB4 and CKA2 genes are reduced in a culture medium, and collecting the objective substance from cells of the yeast and/or the culture medium.
(FR) Cette invention concerne un procédé de production d'une substance visée telle que la phytosphingosine et la sphinganine à l'aide d'une levure. La substance visée est produite par culture d'une levure ayant la capacité de produire la substance visée et modifiée de façon que l'expression et/ou les activités des protéines codées par les gènes LCB4 et CKA2 soient réduites dans un milieu de culture, et collecte de la substance visée à partir des cellules de la levure et/ou du milieu de culture.
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)