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1. (WO2017033152) METHODS FOR DETECTING PHOSPHORYLATED ALPHA-SYNUCLEIN

Pub. No.:    WO/2017/033152    International Application No.:    PCT/IB2016/055088
Publication Date: Fri Mar 03 00:59:59 CET 2017 International Filing Date: Fri Aug 26 01:59:59 CEST 2016
IPC: G01N 33/50
A61K 39/395
C07K 16/18
C07K 16/24
G01N 33/58
Applicants: PROTHENA BIOSCIENCES LIMITED
Inventors: BARBOUR, Robin
ZIESKE, Lynn R.
HAMREN, Sarah
Title: METHODS FOR DETECTING PHOSPHORYLATED ALPHA-SYNUCLEIN
Abstract:
The invention provides methods of detecting alpha-synuclein using a capture antibody and a reporter antibody. The capture antibody binds preferentially to full-length alpha- synuclein phosphorylated at residue 129 (PS129 alpha-synuclein) over unphosphorylated full- length alpha-synuclein. The 11A5 antibody is an example of a suitable capture antibody. The reporter antibody binds to an epitope within residues 40-55 of alpha-synuclein. The 23E8 antibody is an example of such an antibody. Because only a small proportion of alpha-synuclein is phosphorylated high sensitivity of detection below picomolar is advantageous.