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1. (WO2017032808) METHOD FOR GENERATING A RNA-SEQUENCING LIBRARY

Pub. No.:    WO/2017/032808    International Application No.:    PCT/EP2016/069997
Publication Date: Fri Mar 03 00:59:59 CET 2017 International Filing Date: Thu Aug 25 01:59:59 CEST 2016
IPC: C12N 15/10
C12Q 1/68
Applicants: QIAGEN GMBH
Inventors: FANG, Nan
NOLL, Bernhard
HEITZ, Katja
Title: METHOD FOR GENERATING A RNA-SEQUENCING LIBRARY
Abstract:
The invention refers to a novel method of preparing strand-specific RNA-sequencing libraries that can be used to identify DNA coding and non-coding strands that are transcribed to RNA. Such strand-specific RNA-sequencing libraries are especially useful in discovering anti-sense RNA and non-coding RNA. Random primer oligonucleotides, covalently coupled to a moiety, which blocks ligation,are used for RT reaction or the subsequent generation of the second DNA strand so that only one strand of the generated double-stranded DNA is ligated to sequencing adapters at the 5´ nucleotide and sequenced by paired-end sequencing.