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1. (WO2017032342) MOLECULAR WEIGHT INTERNAL STANDARD AND APPLICATIONS THEREOF

Pub. No.:    WO/2017/032342    International Application No.:    PCT/CN2016/096938
Publication Date: Fri Mar 03 00:59:59 CET 2017 International Filing Date: Sat Aug 27 01:59:59 CEST 2016
IPC: C12N 15/11
C12Q 1/68
Applicants: NUHIGH BIOTECHNOLOGIES CO., LTD
苏州新海生物科技股份有限公司
Inventors: BI, Wanli
毕万里
LU, Weihua
陆伟华
Title: MOLECULAR WEIGHT INTERNAL STANDARD AND APPLICATIONS THEREOF
Abstract:
Provided is a molecular weight internal standard, consisting of n nucleotide fragments that have different molecular weights and identifiable markers, the n nucleotide fragments comprising at least p nucleotide fragment pairs differing by one base, and one fragment of the nucleotide fragment pair differing by one base having m bases, and the other fragment having m+1 or m-1 bases; p is 1 to n/2 when n is an even number and p is 1 to (n-1)/2 when n is an odd number. Compared with current commercial molecular weight internal standards, the molecular weight internal standard can be used to identify a single base and differentiate PCR product fragments differing by one base, and is applicable to STR analysis, sequencing, large fragment analysis, and genome analysis.