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1. (WO2017016444) METHOD FOR PREPARING L-2-AMINOBUTYRIC ACID THROUGH BIOCATALYSIS
Latest bibliographic data on file with the International Bureau    Submit observation

Pub. No.: WO/2017/016444 International Application No.: PCT/CN2016/090991
Publication Date: 02.02.2017 International Filing Date: 22.07.2016
IPC:
C12P 13/04 (2006.01) ,C12N 15/53 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
P
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
13
Preparation of nitrogen-containing organic compounds
04
Alpha- or beta-amino acids
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
11
DNA or RNA fragments; Modified forms thereof
52
Genes encoding for enzymes or proenzymes
53
Oxidoreductases (1)
Applicants: ABA CHEMICALS CORPORATION[CN/CN]; 18 Dongfang Rd(E), Port Development Zone, Taicang Port Taicang, Jiangsu 215433, CN
Inventors: LI, Hang; CN
XU, Jun; CN
QUE, Limin; CN
JIANG, Yueheng; CN
CAI, Tong; CN
Agent: LIAN & LIEN IP ATTORNEYS; Room 1207-1208, 12/F, Tower E1, Oriental Plaza, No.1 East Chang An Ave., Dong Cheng District Beijing 100738, CN
Priority Data:
201510443837.824.07.2015CN
Title (EN) METHOD FOR PREPARING L-2-AMINOBUTYRIC ACID THROUGH BIOCATALYSIS
(FR) PROCÉDÉ DE PRÉPARATION D'ACIDE L-2-AMINOBUTYRIQUE PAR BIOCATALYSE
(ZH) 一种生物催化制备L-2-氨基丁酸的方法
Abstract:
(EN) Provided in the present invention is a method for preparing an L-2-aminobutyric acid through biocatalysis, comprising the following steps: constructing a genetically engineered bacteria for simultaneously expressing an FDH and an LDH to obtain a crude enzyme solution containing the FDH and the LDH through fermentation; and performing a biocatalytic reaction in a buffer solution to obtain the L-2-aminobutyric acid. The reaction system comprises a threonine, an ammonium formate, a TD enzyme solution, the FDH/LDH crude enzyme solution, a NAD+ and a pyridoxal phosphate.
(FR) La présente invention concerne un procédé de préparation d'un acide L-2-aminobutyrique par biocatalyse, comprenant les étapes suivantes consistant à : construire une bactérie génétiquement modifiée pour exprimer simultanément une formiate déshydrogénase (FDH) et une lactate déshydrogénase (LDH) pour obtenir une solution d'enzymatique brute contenant une FDH et une LDH par fermentation; et réaliser une réaction biocatalytique dans une solution tampon pour obtenir l'acide L-2-aminobutyrique. Le système réactionnel comprend une thréonine, un formiate d'ammonium, une solution d'enzyme TD, la solution enzymatique brute de FDH/LDH, un NAD+ et un phosphate de pyridoxal.
(ZH) 本发明提供了一种生物催化制备L-2-氨基丁酸的方法,包括如下步骤:构建同时表达FDH和LDH的基因工程菌,经发酵制得含FDH和LDH的粗酶液;在缓冲液中进行生物催化反应制得L-2-氨基丁酸。反应体系包括:苏氨酸、甲酸铵、TD酶液、FDH/LDH粗酶液、NAD +和磷酸吡哆醛。
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: Chinese (ZH)
Filing Language: Chinese (ZH)