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1. WO2017011915 - METHODS AND MICROORGANISMS FOR THE PRODUCTION OF 1,3-BUTANEDIOL

Publication Number WO/2017/011915
Publication Date 26.01.2017
International Application No. PCT/CA2016/050858
International Filing Date 21.07.2016
IPC
C12N 1/21 2006.01
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12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
1Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
20Bacteria; Culture media therefor
21modified by introduction of foreign genetic material
C12N 15/52 2006.01
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15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
11DNA or RNA fragments; Modified forms thereof
52Genes encoding for enzymes or proenzymes
C12N 15/53 2006.01
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15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
11DNA or RNA fragments; Modified forms thereof
52Genes encoding for enzymes or proenzymes
53Oxidoreductases (1)
C12N 15/60 2006.01
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15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
11DNA or RNA fragments; Modified forms thereof
52Genes encoding for enzymes or proenzymes
60Lyases (4)
C12N 9/00 2006.01
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9Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
C12N 9/02 2006.01
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9Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
02Oxidoreductases (1.), e.g. luciferase
CPC
C12N 15/52
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15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
11DNA or RNA fragments; Modified forms thereof
52Genes encoding for enzymes or proenzymes
C12N 9/0006
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9Enzymes; Proenzymes; Compositions thereof
0004Oxidoreductases (1.)
0006acting on CH-OH groups as donors (1.1)
C12N 9/88
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9Enzymes; Proenzymes; Compositions thereof
88Lyases (4.)
C12P 7/18
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PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
7Preparation of oxygen-containing organic compounds
02containing a hydroxy group
04acyclic
18polyhydric
C12P 7/24
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12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
7Preparation of oxygen-containing organic compounds
24containing a carbonyl group
C12Y 101/01001
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12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
YENZYMES
101Oxidoreductases acting on the CH-OH group of donors (1.1)
01with NAD+ or NADP+ as acceptor (1.1.1)
01001Alcohol dehydrogenase (1.1.1.1)
Applicants
  • GOVERNING COUNCIL OF THE UNIVERSITY OF TORONTO [CA]/[CA]
Inventors
  • MAHADEVAN, Radhakrishnan
  • YAKUNIN, Alexander
  • GAWAND, Pratish
  • NEMR, Kayla
Agents
  • NORTON ROSE FULBRIGHT CANADA LLP
Priority Data
62/195,01121.07.2015US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) METHODS AND MICROORGANISMS FOR THE PRODUCTION OF 1,3-BUTANEDIOL
(FR) PROCÉDÉS ET MICRO-ORGANISMES DE PRODUCTION DE 1,3-BUTANEDIOL
Abstract
(EN)
A non-naturally occurring microorganism having a 1,3-BDO pathway is provided. The microorganism expresses at least one of the following 1,3-BDO pathway enzymes: an aldolase that catalyzes condensation of two acetaldehydes to produce 3-hydroxybutanal; and an aldo- ketoreductase, oxidoreductase, aldehyde reductase or alcohol dehydrogenase that reduces 3- hydroxybutanal to 1,3-BDO. The organism may further express one or more enzymes for producing acetaldehyde. A biosynthetic process involves condensing two acetaldehyde molecules to 3-hydroxybutanal using an enzyme from class aldolases; and selectively reducing 3-hydroxybutanal to 1,3-BDO using an enzyme belonging to the class aldo-ketoreductase, oxidoreductase, aldehyde reductase or alcohol dehydrogenase. The process can further include producing acetaldehyde by a biosynthetic method.
(FR)
La présente invention concerne un micro-organisme non d'origine naturelle ayant une voie 1,3-BDO. Le micro-organisme exprime au moins l'une des enzymes suivantes de la voie 1,3-BDO : une aldolase, qui catalyse la condensation de deux acétaldéhydes pour produire du 3-hydroxybutanal; une aldo-cétoréductase, oxydoréductase, aldéhyde réductase ou alcool déshydrogénase, qui réduit le 3-hydroxybutanal en 1,3-BDO. L'organisme peut en outre exprimer une ou plusieurs enzymes de production d'acétaldéhyde. Un procédé de synthèse biologique entraîne la condensation de deux molécules d'acétaldéhyde en 3-hydroxybutanal en utilisant une enzyme de la classe des aldolases; la réduction d'une manière sélective de 3-hydroxybutanal en 1,3-BDO en utilisant une enzyme appartenant à la classe aldo-cétoréductase, oxydoréductase, aldéhyde réductase ou alcool déshydrogénase. Le procédé peut en outre inclure la production d'acétaldéhyde par un procédé de synthèse biologique.
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