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1. WO2017008816 - ENZYMATIC ELECTRODE FOR REGENERATION OF OXIDISED AND/OR REDUCED FORMS OF PYRIDINE NUCLEOTIDES

Publication Number WO/2017/008816
Publication Date 19.01.2017
International Application No. PCT/DK2016/050246
International Filing Date 11.07.2016
IPC
C12Q 1/26 2006.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
26involving oxidoreductase
CPC
C12Q 1/26
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
26involving oxidoreductase
Applicants
  • AARHUS UNIVERSITET [DK]/[DK]
  • UNIVERSITÀ DI ROMA “LA SAPIENZA” [IT]/[IT]
Inventors
  • FERAPONTOVA, Elena
  • BOFFI, Alberto
Agents
  • HØIBERG A/S
Priority Data
PA 2015 7045810.07.2015DK
Publication Language English (en)
Filing Language English (EN)
Designated States
Title
(EN) ENZYMATIC ELECTRODE FOR REGENERATION OF OXIDISED AND/OR REDUCED FORMS OF PYRIDINE NUCLEOTIDES
(FR) ÉLECTRODE ENZYMATIQUE POUR LA RÉGÉNÉRATION DE FORMES OXYDÉES ET/OU RÉDUITES DE NUCLÉOTIDES PYRIDINIQUES
Abstract
(EN) The present invention relates to an electrode for regeneration of oxidised and/or reduced forms of pyridine nucleotides, said electrode characterized by having an electronically conducting surface to which a flavohemoglobin is adsorbed in the presence of an agent that directs enzyme orientation and wherein said flavohemoglobin is coupled to a pyridine nucleotide dependent enzyme. The invention further relates to a biosensor comprising the electrode of the present invention and to uses of said electrode and biosensor.
(FR) La présente invention concerne une électrode pour la régénération de formes oxydées et/ou réduites de nucléotides pyridiniques, ladite électrode étant caractérisée en ce qu'elle présente une surface électroniquement conductrice sur laquelle une flavo-hémoglobine est adsorbée en présence d'un agent qui dirige l'orientation de l'enzyme et ladite flavo-hémoglobine étant couplée à une enzyme dépendante de nucléotides pyridiniques. En outre, l'invention concerne un bio-capteur comprenant l'électrode selon la présente invention et des utilisations de ladite électrode et dudit bio-capteur.
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