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1. (WO2017006859) METHOD FOR DETECTING TARGET NUCLEIC ACID
Latest bibliographic data on file with the International Bureau

Pub. No.: WO/2017/006859 International Application No.: PCT/JP2016/069638
Publication Date: 12.01.2017 International Filing Date: 01.07.2016
IPC:
C12Q 1/68 (2006.01) ,C12M 1/00 (2006.01) ,C12M 1/34 (2006.01) ,C12N 15/09 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
68
involving nucleic acids
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
M
APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
1
Apparatus for enzymology or microbiology
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
M
APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
1
Apparatus for enzymology or microbiology
34
Measuring or testing with condition measuring or sensing means, e.g. colony counters
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
Applicants:
株式会社カネカ KANEKA CORPORATION [JP/JP]; 大阪府大阪市北区中之島二丁目3番18号 3-18, Nakanoshima 2-chome, Kita-ku, Osaka-shi, Osaka 5308288, JP
Inventors:
佐野 創太郎 SANO Sotaro; JP
宮本 重彦 MIYAMOTO Shigehiko; JP
高橋 孝治 TAKAHASHI Koji; JP
Agent:
平木 祐輔 HIRAKI Yusuke; JP
Priority Data:
2015-13465903.07.2015JP
Title (EN) METHOD FOR DETECTING TARGET NUCLEIC ACID
(FR) PROCÉDÉ POUR DÉTECTER UN ACIDE NUCLÉIQUE CIBLE
(JA) 標的核酸の検出法
Abstract:
(EN) The purpose of the present invention is to provide a detection method having a high determination accuracy, said detection method being for detecting a target nucleic acid and comprising conducting a nucleic acid amplification reaction and detecting a nucleic acid amplification product on a solid support, whereby nonspecific detection of a primer dimer can be avoided. A method for detecting a target nucleic acid that comprises conducting a nucleic acid amplification reaction by a Nested-PCR method and detecting a nucleic acid amplification product on a solid support.
(FR) L'objectif de la présente invention concerne un procédé de détection présentant une précision de détermination élevée, ledit procédé de détection étant destiné à détecter un acide nucléique cible et consistant à réaliser une réaction d'amplification d'acide nucléique et à détecter un produit d'amplification d'acide nucléique sur un support solide, la détection non spécifique d'un dimère d'amorce pouvant être évitée. L'invention concerne également un procédé de détection d'un acide nucléique cible qui consiste à réaliser une réaction d'amplification d'acide nucléique par un procédé de PCR nichée et à détecter un produit d'amplification d'acide nucléique sur un support solide.
(JA) 本発明は、核酸増幅反応と固相担体上にて核酸増幅産物を検出することを含む標的核酸の検出方法において、プライマーダイマーの非特異的な検出を回避することを可能とする、判定精度の高い検出方法を提供することを目的とする。 Nested-PCR法による核酸増幅反応と固相担体上にて核酸増幅産物を検出することを含む、標的核酸の検出方法。
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: Japanese (JA)
Filing Language: Japanese (JA)