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1. (WO2016178513) NOVEL GENE INVOLVED IN PRODUCTION OF C5-C8 ORGANIC ACIDS, STRAIN, AND METHOD FOR PREPARING BIOFUEL USING SAME
Latest bibliographic data on file with the International Bureau   

Pub. No.:    WO/2016/178513    International Application No.:    PCT/KR2016/004705
Publication Date: 10.11.2016 International Filing Date: 04.05.2016
IPC:
C12N 9/96 (2006.01), C12N 15/52 (2006.01), C12N 15/74 (2006.01), C12P 7/40 (2006.01)
Applicants: IUCF-HYU (INDUSTRY-UNIVERSITY COOPERATION FOUNDATION HANYANG UNIVERSITY) [KR/KR]; 222, Wangsimni-ro, Seongdong-gu, Seoul 04763 (KR)
Inventors: SANG, Byoung In; (KR).
LEE, Tae Ho; (KR).
JEON, Byoung Seung; (KR)
Agent: JUNG, Eun Youl; (KR)
Priority Data:
10-2015-0063414 06.05.2015 KR
10-2015-0063129 06.05.2015 KR
10-2015-0063128 06.05.2015 KR
10-2015-0063131 06.05.2015 KR
10-2015-0063130 06.05.2015 KR
Title (EN) NOVEL GENE INVOLVED IN PRODUCTION OF C5-C8 ORGANIC ACIDS, STRAIN, AND METHOD FOR PREPARING BIOFUEL USING SAME
(FR) NOUVEAU GÈNE IMPLIQUÉ DANS LA PRODUCTION D'ACIDES ORGANIQUES EN C5-C8, SOUCHE, ET PROCÉDÉ DE PRÉPARATION DE BIOCARBURANT AU MOYEN DE CEUX-CI
(KO) C5-C8 유기산 생산에 관여하는 신규한 유전자, 균주 및 이를 이용한 바이오 연료의 제조방법
Abstract: front page image
(EN)The present invention relates to a novel gene involved in the production of C5-C8 organic acids, a vector comprising the gene, microorganisms transformed with the vector, and a method for producing C5-C8 organic acids using the same and, specifically, to a gene coding an enzyme involved in the biosynthesis of C5-C8 organic acids, the gene comprising at least one nucleotide sequence selected from the group consisting of SEQ ID NO: 1 to SEQ ID NO: 8, and to a method for producing C5-C8 organic acids using the genes. According to the present invention, C5-C8 organic acids can be provided at an improved production yield by providing and using the novel gene involved in the production of C5-C8 organic acids. In addition, the present invention relates to a novel strain producing C5-C8 organic acids and a method for producing C5-C8 organic acids using the same and, specifically, to a Megasphaera hexanoica strain (KCCM11835P) capable of selectively producing C5-C8 organic acids when cultured in a medium containing fructose, and to a method for producing C5-C8 organic acids using the same. The culturing of the strain of the present invention in the medium containing fructose can produce C5-C8 organic acids, and especially, the addition of C2-C6 organic acids from the outside during the culturing can achieve selective conversion production into C5-C8 organic acids. In addition, the produced C5-C8 organic acids can be used in the production of biofuel. In addition, the present invention relates to a method for producing hexanoic acid using a Megasphaera hexanoica strain. The use of the method for producing hexanoic acid according to the present invention can significantly improve the productivity of hexanoic acid. In addition, the present invention relates to a novel strain having C4 and C6 organic acid producing ability, and a method for preparing biofuel using the novel strain and, specifically, to a Caproiciproducens galactitolivorans strain having the ability to produce both C4 organic acids and C6 organic acids when cultured in a carbon source medium, and to a method for preparing biofuel using the same. According to the present invention, a novel strain can be provided that produces both C4 and C6 organic acids through excellent producing ability when cultured in a medium containing carbon sources derived from various biomass or organic waste resources, and, through cultured products of the strain, C8-C12 compounds as high-energy compounds, and biofuel can be produced. In addition, the present invention relates to a novel gene involved in the production of hexanoic acid and a method for preparing hexanoic acid and hexanol using the same and, specifically, to a gene represented by SEQ ID NO: 36 and coding enzymes involved in a metabolic pathway on which hexanoic acid is produced from acetyl-CoA as a start material, to a gene represented by SEQ ID NO: 37 and coding enzymes involved in a metabolic pathway on which pyruvic acid is produced from galactitol as a start material, and to methods for preparing hexanoic acid and hexanol using the genes. According to the present invention, the gene involved in the final production of hexanoic acid using as galactitol as a nutrient is provided, and thus, by using the gene, hexanoic acid, further hexanol, which is one of the main biofuel components, can be provided at an improved production yield.
(FR)La présente invention concerne un nouveau gène impliqué dans la production d'acides organiques en C5-C8, un vecteur comprenant le gène, des micro-organismes transformés avec le vecteur, un procédé de production d'acides organiques en C5-C8 l'utilisant et, plus spécifiquement, un gène codant pour une enzyme impliquée dans la biosynthèse d'acides organiques en C5-C8, le gène comprenant au moins une séquence nucléotidique choisie dans le groupe constitué de SEQ ID n° 1 à SEQ ID n° 8, et un procédé de production d'acides organiques en C5-C8 utilisant les gènes. Selon la présente invention, des acides organiques en C5-C8 peuvent être fournis à un rendement de production amélioré grâce à la fourniture et l'utilisation du nouveau gène impliqué dans la production d'acides organiques en C5-C8. En outre, la présente invention concerne une nouvelle souche produisant des acides organiques en C5-C8 et un procédé de production d'acides organiques en C5-C8 l'utilisant et, spécifiquement, une souche de Megasphaera hexanoica (KCCM11835P) pouvant produire sélectivement des acides organiques en C5-C8 lorsqu'elle est cultivée dans un milieu contenant du fructose, ainsi qu'un procédé de production d'acides organiques en C5-C8 l'utilisant. La culture de la souche de la présente invention dans le milieu contenant du fructose peut produire des acides organiques en C5-C8, et en particulier, l'adjonction d'acides organiques en C2-C6 provenant de l'extérieur pendant la culture permet d'obtenir une production de conversion sélective en acides organiques en C5-C8. En outre, les acides organiques en C5-C8 produits peuvent être utilisés dans la production de biocarburant. En outre, la présente invention concerne un procédé de production d'acide hexanoïque à l'aide d'une souche de Megasphaera hexanoica. L'utilisation du procédé de production d'acide hexanoïque selon la présente invention peut améliorer de façon significative la productivité de l'acide hexanoïque. En outre, la présente invention concerne une nouvelle souche ayant la capacité de production d'acides organiques en C4 et C6, et un procédé de préparation de biocarburant utilisant la nouvelle souche et, spécifiquement, une souche de Caproiciproducens galactitolivorans ayant la capacité de produire à la fois des acides organique en C4 et des acides organiques en C6 lorsqu'elle est mise en culture dans un milieu source de carbone, et un procédé de préparation de biocarburant au moyen de celle-ci. La présente invention concerne une nouvelle souche qui produit à la fois des acides organiques en C4 et en C6 grâce à une excellente capacité de production lorsqu'elle est mise en culture dans un milieu contenant des sources de carbone dérivées de diverses ressources de biomasse ou de déchets organiques, et, grâce à des produits cultivés de la souche, des composés en C8-C12 en tant que composés à haute énergie et du biocarburant peuvent être produits. En outre, la présente invention concerne un nouveau gène impliqué dans la production d'acide hexanoïque et un procédé de préparation d'acide hexanoïque et d'hexanol l'utilisant et, plus spécifiquement, un gène représenté par SEQ ID n° 36 et des enzymes de codage impliquées dans une voie métabolique sur laquelle l'acide hexanoïque est produit à partir d'acétyl-CoA en tant que matière première, un gène représenté par SEQ ID n° 37 et des enzymes de codage impliquées dans la voie métabolique sur laquelle de l'acide pyruvique matière première, et des procédés de préparation d'acide hexanoïque et d'hexanol au moyen des gènes. La présente invention concerne le gène impliqué dans la production finale d'acide hexanoïque à l'aide de galactitol comme nutriment, et ainsi, par l'utilisation dudit gène, de l'acide hexanoïque, puis de l'hexanol, qui est l'un des principaux composants du biocarburant, peuvent être obtenus avec un rendement de production amélioré.
(KO)본 발명은 C5-C8 유기산 생산에 관여하는 신규 유전자, 상기 유전자를 포함하는 벡터, 상기 벡터로 형질전환된 미생물, 및 이를 이용한 C5-C8 유기산 생산방법에 관한 것으로서, 구체적으로는 서열번호 1 내지 8로 이루어진 군으로부터 선택된 하나 이상의 염기서열을 포함하는, C5-C8 유기산 생합성에 관여하는 효소를 코딩하는 유전자, 및 상기 유전자들을 이용한 C5-C8 유기산을 생산하는 방법에 관한 것이다. 본 발명에 따르면, C5-C8 유기산 생산에 관여하는 신규 유전자를 제공함으로써, 이를 이용하여 C5-C8 유기산을 향상된 생산 수율로 제공할 수 있다. 또한, 본 발명은 C5-C8 유기산을 생산하는 신규 균주 및 이를 이용하여 C5-C8 유기산을 생산하는 방법에 관한 것으로서, 구체적으로는 프룩토스를 함유하는 배지에서 배양시 C5-C8 유기산을 선택적으로 생산할 수 있는 메가스페라 헥사노이카(Megasphaera hexanoica) 균주(KCCM11835P) 및 이를 이용한 C5-C8 유기산을 생산하는 방법에 관한 것이다. 본 발명의 균주를 프룩토스를 포함하는 배지에서 배양할 경우 C5-C8 유기산을 생산할 수 있으며, 특히 배양하는 동안 외부에서 C2-C6 유기산을 첨가할 경우 C5-C8 유기산으로 선택적 전환생산이 가능하다. 또한 생산된 C5-C8 유기산은 바이오 연료의 생산에 이용될 수 있다. 또한, 본 발명은 메가스페라 헥사노이카 균주를 이용하여 헥사노익산을 생산하는 방법에 관한 것이다. 본 발명에 따른 헥사노익산 생산방법을 이용하면, 헥사노익산의 생산성을 획기적으로 향상시킬 수 있다. 또한, 본 발명은 C4 및 C6 유기산 생산능을 갖는 신규 균주 및 상기 신규 균주를 이용한 바이오 연료의 제조방법에 관한 것으로서, 구체적으로는, 탄소원 배지 중에서 배양시 C4 유기산 및 C6 유기산의 동시 생산 능력을 갖는 카프로이시프로두센스 갈락티토리보란스 (Caproiciproducens galactitolivorans) 균주 및 이를 이용한 바이오 연료의 제조방법에 관한 것이다. 본 발명에 따르면, 다양한 바이오매스 또는 유기성 폐자원으로부터 유래된 탄소원을 함유한 배지 중에서 배양될 경우, 우수한 생산능으로 C4 및 C6 유기산을 동시생산하는 신규 균주를 제공하며, 상기 균주의 배양 산물을 통해서 고에너지 화합물인 C8-C12 화합물 및 바이오연료를 생산해낼 수 있다. 또한, 본 발명은 본 발명은 헥사노익산 생산에 관여하는 신규 유전자 및 이를 이용한 헥사노익산 및 헥산올의 제조방법에 관한 것으로서, 구체적으로는, 아세틸-CoA를 출발 물질로 하여 헥사노익산을 생산하는 대사경로에 관여하는 효소들을 암호화하는 서열번호 36으로 표시되는 유전자, 갈락티톨을 출발 물질로 하여 피루브산을 생산하는 대사경로에 관여하는 효소들을 암호화하는 서열번호 37로 표시되는 유전자, 및 상기 유전자들을 이용한 헥사노익산 및 헥산올의 제조방법에 관한 것이다. 본 발명에 따르면, 갈락티톨을 영양원으로 하여 최종적으로 헥사노익산을 생산하는데 관여하는 유전자를 제공함으로써, 이를 이용하여 헥사노익산, 더 나아가 주요 바이오 연료 성분 중 하나인 헥산올을 더욱 향상된 생산 수율로 제공할 수 있다.
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KN, KP, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW.
African Regional Intellectual Property Organization (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG).
Publication Language: Korean (KO)
Filing Language: Korean (KO)