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Pub. No.: WO/2016/175451 International Application No.: PCT/KR2016/002545
Publication Date: 03.11.2016 International Filing Date: 15.03.2016
IPC:
C12Q 1/24 (2006.01) ,C12N 5/09 (2010.01) ,C12M 1/12 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
02
involving viable micro-organisms
24
Methods of sampling, or inoculating or spreading a sample; Methods of physically isolating an intact micro-organism
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5
Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
09
Tumour cells
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
M
APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
1
Apparatus for enzymology or microbiology
12
with sterilisation, filtration, or dialysis means
Applicants: CYTOGEN CO.,LTD[KR/KR]; A204(Moonjung-dong)52, Chungmin-ro Songpa-gu Seoul 05839, KR
Inventors: JEON, Byung Hee; KR
Agent: HAN, Sang Soo; KR
Priority Data:
10-2015-006109130.04.2015KR
Title (EN) TARGET CELL COLLECTION METHOD
(FR) PROCÉDÉ DE COLLECTE DE CELLULES CIBLES
(KO) 표적 세포 회수 방법
Abstract:
(EN) Disclosed is a target cell collection method, which separates target cells from a fluid sample such as blood and physiological fluid, and then effectively collects the separated target cells while preventing damage thereto. The target cell collection method according to the present invention collects target cells separated from a cell mixed liquid and comprises the steps of: moving a bio chip, on which the separated target cells are located, into a container; primarily injecting a buffer into the bio chip; and primarily sucking the buffer from a first area within the bio chip so as to collect the buffer in a collection space.
(FR) L'invention concerne un procédé de collecte de cellules cibles, qui consiste à séparer des cellules cibles à partir d'un échantillon de fluide, tel que du sang ou un liquide physiologique, puis à collecter efficacement les cellules cibles séparées tout en empêchant leur endommagement. Le procédé de collecte de cellules cibles selon la présente invention consiste à collecter des cellules cibles séparées à partir d'un mélange de cellules liquide, et comprend les étapes consistant à : transférer une puce biologique, sur laquelle les cellules cibles séparées sont placées, dans un contenant ; injecter principalement un tampon dans la puce biologique ; et aspirer principalement le tampon à partir d'une première zone à l'intérieur de la puce biologique, de manière à collecter le tampon dans un espace de collecte.
(KO) 혈액, 생리학적 유체 등의 유체샘플로부터 표적 세포를 분리한 후, 분리된 표적 세포의 손상을 방지하면서 효율적으로 회수하는 표적 세포 회수 방법이 개시된다. 본 발명에 따른 표적 세포 회수 방법은, 세포 혼합액에서 분리된 표적 세포를 회수하는 방법에 있어서, 상기 분리된 표적 세포가 위치한 바이오 칩(Bio Chip)을, 용기의 내부로 이동시키는 단계, 버퍼(buffer)를 상기 바이오 칩 내부에 1차 주입시키는 단계 및 상기 바이오 칩 내부의 제 1 영역에서 상기 버퍼를 1차적으로 흡입하여 회수공간에 회수하는 단계를 포함하는 것을 특징으로 한다.
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KN, KP, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: Korean (KO)
Filing Language: Korean (KO)