Processing

Please wait...

Settings

Settings

Goto Application

1. WO2016168681 - ITERATIVE PLATFORM FOR THE SYNTHESIS OF ALPHA FUNCTIONALIZED PRODUCTS

Publication Number WO/2016/168681
Publication Date 20.10.2016
International Application No. PCT/US2016/027873
International Filing Date 15.04.2016
IPC
C12N 1/20 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
1Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
20Bacteria; Culture media therefor
C12N 1/21 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
1Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
20Bacteria; Culture media therefor
21modified by introduction of foreign genetic material
C12P 7/44 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
7Preparation of oxygen-containing organic compounds
40containing a carboxyl group
44Polycarboxylic acids
A61K 38/45 2006.01
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
38Medicinal preparations containing peptides
16Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
43Enzymes; Proenzymes; Derivatives thereof
45Transferases (2)
A61K 38/51 2006.01
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
38Medicinal preparations containing peptides
16Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
43Enzymes; Proenzymes; Derivatives thereof
51Lyases (4)
CPC
A61K 35/00
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
35Medicinal preparations containing materials or reaction products thereof with undetermined constitution
A61K 38/00
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
38Medicinal preparations containing peptides
C12N 15/52
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
11DNA or RNA fragments; Modified forms thereof
52Genes encoding for enzymes or proenzymes
C12N 9/0006
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes; Proenzymes; Compositions thereof
0004Oxidoreductases (1.)
0006acting on CH-OH groups as donors (1.1)
C12N 9/0008
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes; Proenzymes; Compositions thereof
0004Oxidoreductases (1.)
0008acting on the aldehyde or oxo group of donors (1.2)
C12N 9/001
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes; Proenzymes; Compositions thereof
0004Oxidoreductases (1.)
001acting on the CH-CH group of donors (1.3)
Applicants
  • WILLIAM MARSH RICE UNIVERSITY [US]/[US]
Inventors
  • GONZALEZ, Ramon
  • CLOMBURG, James, M.
  • CHEONG, Seokjung
Agents
  • VALOIR, Tamsen
Priority Data
62/148,12315.04.2015US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) ITERATIVE PLATFORM FOR THE SYNTHESIS OF ALPHA FUNCTIONALIZED PRODUCTS
(FR) PLATE-FORME ITÉRATIVE POUR LA SYNTHÈSE DE PRODUITS FONCTIONNALISÉS EN ALPHA
Abstract
(EN)
The use of microorganisms to make alpha-functionalized chemicals and fuels, (e.g. alpha- functionalized carboxylic acids, alcohols, hydrocarbons, amines, and their beta-, and omega- functionalized derivatives), by utilizing an iterative carbon chain elongation pathway that uses functionalized extender units. The core enzymes in the pathway include thiolase, dehydrogenase, dehydratase and reductase. Native or engineered thiolases catalyze the condensation of either unsubstituted or functionalized acyl-CoA primers with an alpha- functionalized acetyl-CoA as the extender unit to generate alpha-functionalized β-keto acyl- CoA. Dehydrogenase converts alpha-functionalized β-keto acyl-CoA to alpha-functionalized β-hydroxy acyl-CoA. Dehydratase converts alpha-functionalized β-hydroxy acyl-CoA to alpha-functionalized enoyl-CoA. Reductase converts alpha-functionalized enoyl-CoA to alpha-functionalized acyl-CoA. The platform can be operated in an iterative manner (i.e. multiple turns) by using the resulting alpha-functionalized acyl-CoA as primer and the aforementioned alpha-functionalized extender unit in subsequent turns of the cycle. Termination pathways acting on any of the four alpha-functionalized CoA thioester intermediates terminate the platform and generate various alpha-functionalized carboxylic acids, alcohols and amines with different β-reduction degree.
(FR)
La présente invention concerne l'utilisation de micro-organismes pour préparer des produits chimiques fonctionnalisés en alpha et des carburants, (par exemple des acides carboxyliques, alcools, hydrocarbures, amines fonctionnalisés en alpha ainsi que leurs dérivés fonctionnalisés en bêta et en oméga), en utilisant une voie d'allongement de chaine carbonée itérative qui utilise des motifs d'extension fonctionnalisés. Les enzymes centrales dans ladite voie comprennent les thiolase, déshydrogénase, déshydratase et réductase. Les thiolases natives ou modifiées catalysent la condensation d'amorces d'acyl-CoA soit non substituées soit fonctionnalisées, un acétyl-CoA fonctionnalisé en alpha étant utilisé comme motif d'extension permettant de générer un β-céto-acyl-CoA fonctionnalisé en alpha. La déshydrogénase convertit le β-céto-acyl-CoA fonctionnalisé en alpha en un β-hydroxy-acyl-CoA fonctionnalisé en alpha. La déshydratase convertit un β-hydroxy-acyl-CoA fonctionnalisé en alpha en un énoyl-CoA fonctionnalisé en alpha. La réductase convertit un énoyl-CoA fonctionnalisé en alpha en un acyl-CoA fonctionnalisé en alpha. La plate-forme peut être actionnée de manière itérative (c'est-à-dire de multiples fois) en utilisant l'acyl-CoA fonctionnalisé en alpha ainsi obtenu comme amorce et ledit motif d'extension fonctionnalisé en alpha précité au cours des tours suivants du cycle. Des voies de terminaison, agissant sur l'un quelconque des quatre intermédiaires de thioester de CoA fonctionnalisé en alpha, terminent la plate-forme et génèrent divers acides carboxyliques, alcools et amines fonctionnalisés en alpha présentant différents degrés de réduction en bêta.
Also published as
Latest bibliographic data on file with the International Bureau