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1. WO2016161043 - BIOCONVERSION OF SHORT-CHAIN HYDROCARBONS TO FUELS AND CHEMICALS

Publication Number WO/2016/161043
Publication Date 06.10.2016
International Application No. PCT/US2016/025103
International Filing Date 31.03.2016
IPC
C12N 15/70 2006.01
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12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
70Vectors or expression systems specially adapted for E. coli
C12N 9/00 2006.01
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NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
C12P 7/42 2006.01
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PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
7Preparation of oxygen-containing organic compounds
40containing a carboxyl group
42Hydroxy carboxylic acids
C12P 7/44 2006.01
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PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
7Preparation of oxygen-containing organic compounds
40containing a carboxyl group
44Polycarboxylic acids
CPC
C12N 9/00
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9Enzymes; Proenzymes; Compositions thereof
C12N 9/0006
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9Enzymes; Proenzymes; Compositions thereof
0004Oxidoreductases (1.)
0006acting on CH-OH groups as donors (1.1)
C12N 9/0008
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9Enzymes; Proenzymes; Compositions thereof
0004Oxidoreductases (1.)
0008acting on the aldehyde or oxo group of donors (1.2)
C12N 9/001
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9Enzymes; Proenzymes; Compositions thereof
0004Oxidoreductases (1.)
001acting on the CH-CH group of donors (1.3)
C12N 9/0077
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9Enzymes; Proenzymes; Compositions thereof
0004Oxidoreductases (1.)
0071acting on paired donors with incorporation of molecular oxygen (1.14)
0077with a reduced iron-sulfur protein as one donor (1.14.15)
C12N 9/13
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9Enzymes; Proenzymes; Compositions thereof
10Transferases (2.)
13transferring sulfur containing groups (2.8)
Applicants
  • WILLIAM MARSH RICE UNIVERSITY [US]/[US]
Inventors
  • GONZALEZ, Ramon
  • CLOMBURG, James
Agents
  • VALOIR, Tamsen
Priority Data
62/140,62831.03.2015US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) BIOCONVERSION OF SHORT-CHAIN HYDROCARBONS TO FUELS AND CHEMICALS
(FR) BIOCONVERSION D'HYDROCARBURES À CHAÎNE COURTE EN CARBURANTS ET PRODUITS CHIMIQUES
Abstract
(EN)
An engineered microorganism(s) with novel pathways for the conversion of short- chain hydrocarbons to fuels and chemicals (e.g. carboxylic acids, alcohols, hydrocarbons, and their alpha-, beta-, and omega-functionalized derivatives) is described. Key to this approach is the use of hydrocarbon activation enzymes able to overcome the high stability and low reactivity of hydrocarbon compounds through the cleavage of an inert C-H bond. Oxygen-dependent or oxygen-independent activation enzymes can be exploited for this purpose, which when combined with appropriate pathways for the conversion of activated hydrocarbons to key metabolic intermediates, enables the generation of product precursors that can subsequently be converted to desired compounds through established pathways. These novel engineered microorganism(s) provide a route for the production of fuels and chemicals from short chain hydrocarbons such as methane, ethane, propane, butane, and pentane.
(FR)
L'invention concerne un ou des microorganismes obtenus par génie génétique comportant de nouvelles voies pour la conversion d'hydrocarbures à chaîne courte en carburants et en produits chimiques (par exemple des acides carboxyliques, des alcools, des hydrocarbures, et leurs dérivés fonctionnalisés en alpha, bêta et oméga). L'élément clé dans cette démarche est l'utilisation d'enzymes d'activation des hydrocarbures capables de venir à bout de la stabilité élevée et de la faible réactivité des composés d'hydrocarbures par clivage d'une liaison C-H inerte. Des enzymes d'activation dépendantes ou pas de l'oxygène peuvent être exploitées à cet effet : lorsqu'elles sont combinées à des voies appropriées pour la conversion d'hydrocarbures activés en intermédiaires métaboliques clés, celles-ci permettent la génération de précurseurs de produit qui peuvent ensuite être convertis en composés désirés par des voies déjà connues. Ce ou ces nouveaux microorganismes obtenus par génie génétique permettent la production de carburants et de produits chimiques à partir d'hydrocarbures à chaîne courte tels que le méthane, l'éthane, le propane, le butane et le pentane.
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