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1. WO2016148179 - METHOD FOR PREPARING DENDRITIC CELL BY NON-ADHESIVE CULTURE USING IFN

Publication Number WO/2016/148179
Publication Date 22.09.2016
International Application No. PCT/JP2016/058295
International Filing Date 16.03.2016
Chapter 2 Demand Filed 14.09.2016
IPC
C12N 5/0784 2010.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
07Animal cells or tissues
071Vertebrate cells or tissues, e.g. human cells or tissues
078Cells from blood or from the immune system
0784Dendritic cells; Progenitors thereof
A61K 35/15 2015.1
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
35Medicinal preparations containing materials or reaction products thereof with undetermined constitution
12Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
14Blood; Artificial blood
15Cells of the myeloid line, e.g. granulocytes, basophils, eosinophils, neutrophils, leucocytes, monocytes, macrophages or mast cells; Myeloid precursor cells; Antigen-presenting cells, e.g. dendritic cells
A61P 35/00 2006.1
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
35Antineoplastic agents
A61P 35/02 2006.1
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
35Antineoplastic agents
02specific for leukemia
A61P 37/02 2006.1
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
37Drugs for immunological or allergic disorders
02Immunomodulators
A61P 43/00 2006.1
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
43Drugs for specific purposes, not provided for in groups A61P1/-A61P41/108
CPC
A61K 2039/5154
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
39Medicinal preparations containing antigens or antibodies
51comprising whole cells, viruses or DNA/RNA
515Animal cells
5154Antigen presenting cells [APCs], e.g. dendritic cells, macrophages
A61K 2039/5158
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
39Medicinal preparations containing antigens or antibodies
51comprising whole cells, viruses or DNA/RNA
515Animal cells
5158Antigen-pulsed cells, e.g. T-cells
A61K 35/13
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
35Medicinal preparations containing materials or reaction products thereof with undetermined constitution
12Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
13Tumour cells, irrespective of tissue of origin
A61K 35/15
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
35Medicinal preparations containing materials or reaction products thereof with undetermined constitution
12Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
14Blood; Artificial blood
15Cells of the myeloid line, e.g. granulocytes, basophils, eosinophils, neutrophils, leucocytes, monocytes, macrophages or mast cells; Myeloid precursor cells; Antigen-presenting cells, e.g. dendritic cells
A61K 35/17
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
35Medicinal preparations containing materials or reaction products thereof with undetermined constitution
12Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
14Blood; Artificial blood
17Lymphocytes; B-cells; T-cells; Natural killer cells; Interferon-activated or cytokine-activated lymphocytes
A61K 39/001139
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
39Medicinal preparations containing antigens or antibodies
0005Vertebrate antigens
0011Cancer antigens
001136Cytokines
001139Colony stimulating factors [CSF]
Applicants
  • 国立大学法人信州大学 SHINSHU UNIVERSITY [JP]/[JP]
Inventors
  • 下平 滋隆 SHIMODAIRA Shigetaka
  • 小屋 照継 KOYA Terutsugu
Agents
  • 平木 祐輔 HIRAKI Yusuke
Priority Data
2015-05380417.03.2015JP
Publication Language Japanese (ja)
Filing Language Japanese (JA)
Designated States
Title
(EN) METHOD FOR PREPARING DENDRITIC CELL BY NON-ADHESIVE CULTURE USING IFN
(FR) MÉTHODE DE PRÉPARATION DE CELLULES DENDRITIQUES PAR CULTURE NON-ADHÉSIVE UTILISANT DE L'IFN
(JA) IFNを用いた非接着培養による樹状細胞の調製方法
Abstract
(EN) The present invention addresses the problem of providing a method for preparing a dendritic cell from a monocyte by a non-adhesive culture method using interferon-α. The present invention is a method for preparing a dendritic cell having cytotoxicity from a monocyte, said method comprising culturing a monocyte isolated from peripheral blood by non-adhesive culture in the presence of GM-CSF and pegylated interferon-α, then adding prostaglandin E2 and OK432 to the resultant culture, and then further carrying out the culturing by non-adhesive culture.
(FR) La présente invention aborde le problème consistant à fournir une méthode de préparation de cellules dendritiques à partir de monocytes à l'aide d'une méthode de culture non-adhésive utilisant de l'interféron-α. La présente invention concerne une méthode de préparation de cellules dendritiques à activité cytotoxique à partir de monocytes, ladite méthode comprenant l'action de cultiver des monocytes isolés à partir de sang périphérique en culture non-adhésive en présence de GM-CSF et d'interféron-α pégylé, puis ajouter la prostaglandine E2 et OK432 à la culture résultante, puis ensuite continuer de cultiver les cellules en culture non-adhésive.
(JA) 本発明は、インターフェロンαを用いて単球から非接着培養法により樹状細胞を調製する方法の提供を目的とし、本発明は末梢血より単離した単球を、GM-CSF及びPEG化インターフェロンαの存在下で非接着培養により培養し、その後、プロスタグランジンE2及びOK432を添加してさらに非接着培養により培養することを含む、単球から細胞傷害性を有する樹状細胞を調製する方法である。
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