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1. WO2016138289 - METHODS OF GENERATING CELLS OF THE SMOOTH MUSCLE LINEAGE AND USES THEREOF

Publication Number WO/2016/138289
Publication Date 01.09.2016
International Application No. PCT/US2016/019605
International Filing Date 25.02.2016
IPC
C12N 5/00 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
C12N 5/02 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
02Propagation of single cells or cells in suspension; Maintenance thereof; Culture media therefor
CPC
C12N 2501/727
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
2501Active agents used in cell culture processes, e.g. differentation
70Enzymes
72Transferases (EC 2.)
727Kinases (EC 2.7.)
C12N 2506/02
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
2506Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
02from embryonic cells
C12N 2506/45
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
2506Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
45from artificially induced pluripotent stem cells
C12N 5/0661
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor;
06Animal cells or tissues; Human cells or tissues
0602Vertebrate cells
0652Cells of skeletal and connective tissues; Mesenchyme
0661Smooth muscle cells
Applicants
  • THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIVERSITY [US]/[US]
Inventors
  • REIJO PERA, Renee A.
  • CHEN, Bertha
  • BAER, Thomas M.
  • PHADNIS, Smruti
  • WEN, Yan
Agents
  • GUEDELHOEFER, Otto C.
Priority Data
62/126,33327.02.2015US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) METHODS OF GENERATING CELLS OF THE SMOOTH MUSCLE LINEAGE AND USES THEREOF
(FR) PROCÉDÉS DE GÉNÉRATION DE CELLULES DE LA LIGNÉE DES MUSCLES LISSES ET LEURS UTILISATIONS
Abstract
(EN)
Methods are provided for generating a homogenous population of smooth muscle progenitor cells from pluripotent progenitor cells and treating a subject having a smooth muscle defect, deficiency or disease through the use of such cells. Methods are also provided for treating a subject for a smooth muscle deficiency, defect, and/or disease with cellular therapy using homogenous populations of smooth muscle precursors and/or differentiated smooth muscle cells derived according to the methods described herein. Some aspects of the treatment include methods of generating homogenous populations of smooth muscle precursor cells and/or differentiated smooth muscle cells from pluripotent progenitor cells using non-invasive automated identification of cell types based on cellular behavior parameters of cultured pluripotent progenitor cells and removal and/or isolation of the identified cell types. Also provided are systems, compositions, and kits for practicing the methods of the disclosure.
(FR)
L'invention concerne des procédés pour générer une population homogène de cellules progénitrices des muscles lisses à partir de cellules progénitrices pluripotentes et pour traiter un sujet présentant un défaut, une déficience ou une maladie des muscles lisses grâce à l'utilisation de telles cellules. L'invention concerne également des procédés pour traiter un sujet souffrant d'une déficience, d'un défaut et/ou d'une maladie des muscles lisses par une thérapie cellulaire faisant appel à des populations homogènes de précurseurs des muscles lisses et/ou de cellules différenciées des muscles lisses dérivées selon les procédés décrits dans la description. Certains aspects du traitement comprennent des procédés consistant à générer des populations homogènes de cellules précurseurs des muscles lisses et/ou des cellules différenciées des muscles lisses à partir de cellules progénitrices pluripotentes faisant appel à une identification automatique non invasive de types cellulaires sur base de paramètres de comportement cellulaire de cellules progénitrices pluripotentes cultivées et à l'élimination et/ou à l'isolement des types cellulaires identifiés. L'invention concerne également des systèmes, des compositions et des kits pour la mise en oeuvre des procédés selon l'invention.
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