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1. WO2016138015 - ENZYME COUPLED ASSAY FOR QUANTIFICATION OF PROTEIN AND PEPTIDE BINDING BY SAMDI MASS SPECTROMETRY

Publication Number WO/2016/138015
Publication Date 01.09.2016
International Application No. PCT/US2016/019163
International Filing Date 23.02.2016
IPC
G01N 33/68 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
68involving proteins, peptides or amino acids
G01N 33/573 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
573for enzymes or isoenzymes
CPC
G01N 2333/47
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
2333Assays involving biological materials from specific organisms or of a specific nature
435from animals; from humans
46from vertebrates
47Assays involving proteins of known structure or function as defined in the subgroups
G01N 2333/98
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
2333Assays involving biological materials from specific organisms or of a specific nature
90Enzymes; Proenzymes
914Hydrolases (3)
978acting on carbon to nitrogen bonds other than peptide bonds (3.5)
98acting on amide bonds in linear amides (3.5.1)
G01N 2560/00
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
2560Chemical aspects of mass spectrometric analysis of biological material
G01N 33/54306
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
543with an insoluble carrier for immobilising immunochemicals
54306Solid-phase reaction mechanisms
G01N 33/54353
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
543with an insoluble carrier for immobilising immunochemicals
54353with ligand attached to the carrier via a chemical coupling agent
Applicants
  • NORTHWESTERN UNIVERSITY [US]/[US]
Inventors
  • MRKSICH, Milan
  • O'KANE, Patrick
Agents
  • ALLIKIAN, Michael, J.
Priority Data
62/119,40923.02.2015US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) ENZYME COUPLED ASSAY FOR QUANTIFICATION OF PROTEIN AND PEPTIDE BINDING BY SAMDI MASS SPECTROMETRY
(FR) DOSAGE DE COUPLAGE ENZYMATIQUE POUR LA QUANTIFICATION D'UNE LIAISON DE PROTÉINE ET DE PEPTIDE PAR SPECTROMÉTRIE DE MASSE SAMDI
Abstract
(EN)
Disclosed herein are methods of using an immobilized substrate, immobilized ligand, and a fusion protein of an enzyme for the substrate and a receptor for the ligand, where the immobilized substrate can react to form an immobilized product that has a different mass than the immobilized substrate, and using this transformation to indirectly determine the binding of the receptor and the ligand. These methods can be used for high-throughput screening for possible modulators (e.g., inhibitors or activators) of the ligand -receptor interaction.
(FR)
L'invention concerne des procédés consistant à utiliser un substrat immobilisé, un ligand immobilisé, et une protéine de fusion d'une enzyme pour le substrat et un récepteur pour le ligand, le substrat immobilisé pouvant réagir pour former un produit immobilisé qui a une masse différente de celle du substrat immobilisé, et à utiliser cette transformation pour déterminer indirectement la liaison du récepteur et du ligand. Ces procédés peuvent être utilisés pour le criblage à haut rendement pour des modulateurs éventuels (par exemple, des inhibiteurs ou des activateurs) de l'interaction ligand-récepteur.
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