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1. WO2016137236 - METHOD FOR DETECTING VIRUS BY USING LIPOSOME OR LIPOSOME-POLYMER HYBRID

Publication Number WO/2016/137236
Publication Date 01.09.2016
International Application No. PCT/KR2016/001829
International Filing Date 25.02.2016
IPC
G01N 33/569 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
569for microorganisms, e.g. protozoa, bacteria, viruses
G01N 33/535 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
531Production of immunochemical test materials
532Production of labelled immunochemicals
535with enzyme label
G01N 33/52 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
52Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper
CPC
G01N 33/52
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
52Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper ; and including single- and multilayer analytical elements
G01N 33/535
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
531Production of immunochemical test materials
532Production of labelled immunochemicals
535with enzyme label ; or co-enzymes, co-factors, enzyme inhibitors or enzyme substrates
G01N 33/569
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
569for microorganisms, e.g. protozoa, bacteria, viruses
Applicants
  • 재단법인 바이오나노헬스가드연구단 BIONANO HEALTH GUARD RESEARCH CENTER [KR]/[KR]
Inventors
  • 정봉현 CHUNG, Bong Hyun
  • 배판기 BAE, Pan Kee
  • 박경미 PARK, Kyung Mi
  • 김유정 KIM, Yujeong
Agents
  • 이처영 LEE, Cheo Young
Priority Data
10-2015-002655625.02.2015KR
10-2016-002205724.02.2016KR
Publication Language Korean (KO)
Filing Language Korean (KO)
Designated States
Title
(EN) METHOD FOR DETECTING VIRUS BY USING LIPOSOME OR LIPOSOME-POLYMER HYBRID
(FR) PROCÉDÉ DE DÉTECTION DE VIRUS UTILISANT UN LIPOSOME OU UN HYBRIDE LIPOSOME-POLYMÈRE
(KO) 리포좀 또는 리포좀-폴리머 하이브리드를 이용한 바이러스 검출방법
Abstract
(EN)
The present invention relates to a method for detecting a virus by using a liposome or a liposome-polymer hybrid and, more specifically, to a method for detecting a virus by making a liposome or a liposome-polymer hybrid, both of which contain a chromogenic substrate, come into contact with a sample assumed to contain a virus, and then detecting whether there is a virus, through color development according to whether there is an emission of the chromogenic substrate, which is contained in the liposome or the liposome-polymer hybrid, by the contact made therebetween. According to the present invention, the method for detecting a virus by using a liposome or a liposome-polymer hybrid allows a virus to be easily detected with the naked eye. Particularly, when using a liposome or a liposome-polymer hybrid, both of which contain hydrophobic 3,3',5,5'-tetramethyl bezidine (TMB) or iodine, which are chromogenic substrates, the stability to natural color development by oxygen or a chemical reaction is high since TMB or iodine does not leak to the outside of the liposome or the liposome-polymer hybrid. Additionally, it was shown that TMB or iodine contained in the liposome or the liposome-polymer hybrid is discharged and a virus detection signal to be generated by a reaction with HRP or starch, both of which are color development inducer materials, is remarkably high when a virus binds to the liposome or the liposome-polymer hybrid through a viral membrane protein.
(FR)
La présente invention concerne un procédé de détection d'un virus en utilisant un liposome ou un hybride liposome-polymère et, plus spécifiquement, un procédé de détection d'un virus par fabrication d'un liposome ou d'un hybride liposome-polymère, les deux contenant un substrat chromogène, entrant en contact avec un échantillon supposé contenir un virus, puis détecter la présence ou l'absence d'un virus, à travers le développement de couleurs selon qu'il existe ou non une émission du substrat chromogène, qui est contenu dans le liposome ou l'hybride liposome-polymère, par le contact réalisé entre les deux. Selon la présente invention, le procédé de détection d'un virus en utilisant un liposome ou un hybride liposome-polymère permet la détection facile d'un virus à l'œil nu. En particulier, lors de l'utilisation d'un liposome ou d'un hybride liposome-polymère, les deux contenant de la 3,3',5,5'-tétraméthyl bézidine (TMB) ou de l'iode hydrophobe, qui sont des substrats chromogènes, la stabilité de développement de couleur naturelle par l'oxygène ou une réaction chimique est élevée étant donné que la TMB ou l'iode ne fuit pas à l'extérieur du liposome ou de l'hybride liposome-polymère. De plus, on a démontré que la TMB ou l'iode contenu dans le liposome ou l'hybride liposome-polymère est déchargé et que le signal de détection de virus devant être produit par une réaction avec HRP ou de l'amidon, qui sont tous deux des matériaux inducteurs de développement de couleur, est remarquablement élevé lorsqu'un virus se lie au liposome ou à l'hybride liposome-polymère par le biais d'une protéine de membrane virale.
(KO)
본 발명은 리포좀 또는 리포좀-폴리머 하이브리드(liposome-polymer hybrid)를 이용한 바이러스 검출방법에 관한 것으로, 더욱 자세하게는 발색기질이 내포된 리포좀 또는 리포좀-폴리머 하이브리드를 바이러스를 함유하는 것으로 추정되는 시료와 접촉시킨 다음, 상기 접촉으로 리포좀 또는 리포좀-폴리머 하이브리드에 내포된 발색기질의 방출 유무에 따른 발색을 통해 바이러스의 유무를 검출하는 것을 특징으로 하는 바이러스 검출방법에 관한 것이다. 본 발명에 따른 리포좀 또는 리포좀-폴리머 하이브리드를 이용한 바이러스의 검출방법은 바이러스를 육안으로 쉽게 검출 가능하고, 특히 발색기질인 소수성 TMB(3,3',5,5'-tetramethyl bezidine) 또는 아이오딘(iodine)을 내포하는 리포좀 또는 리포좀-폴리머 하이브리드를 이용할 경우 TMB 또는 아이오딘이 리포좀 또는 리포좀-폴리머 하이브리드 밖으로 유출되지 않아 산소 또는 화학반응에 의한 자연발색에 대한 안정성이 높고, 상기 리포좀 또는 리포좀-폴리머 하이브리드에 바이러스가 바이러스 막단백질을 통해 결합할 경우, 리포좀 또는 리포좀-폴리머 하이브리드에 내포된 TMB 또는 아이오딘(iodine)이 방출되고 발색유도물질인 HRP 또는 전분(starch)과 반응하여 나타나는 바이러스 검출신호가 현저히 높은 것으로 나타났다.
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