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1. (WO2016106033) PROTEINASE-FREE COATINGS FOR COLONY PASSAGING
Latest bibliographic data on file with the International Bureau   

Pub. No.:    WO/2016/106033    International Application No.:    PCT/US2015/065957
Publication Date: 30.06.2016 International Filing Date: 16.12.2015
IPC:
C12N 5/00 (2006.01)
Applicants: CORNING INCORPORATED [US/US]; 1 Riverfront Plaza Corning, New York 14831 (US)
Inventors: HENRY, David; (FR).
HERVY, Martial; (FR).
WALERACK, Corinne; (FR).
ZHOU, Yue; (US).
FANG, Ye; (US).
FERRIE, Ann MeeJin; (US).
GORAL, Vasiliy Nikoleavich; (US)
Agent: WILKS, Susan S; (US)
Priority Data:
62/095,147 22.12.2014 US
Title (EN) PROTEINASE-FREE COATINGS FOR COLONY PASSAGING
(FR) REVÊTEMENTS EXEMPTS DE PROTÉINASE POUR LE PASSAGE DE COLONIES
Abstract: front page image
(EN)A cell culture article includes a substrate having a polymer coating that is conducive to colony passaging of cells cultured on the coating. Example polymer coatings are formed from polygalacturonic acid (PGA), alginate, or combinations thereof. Cells cultured on the polymer coating can be separated from the substrate as a colony or layer of cells by exposing the polymer coating to (i) a chelating agent, (ii) a proteinase-free enzyme, or (iii) a chelating agent and a proteinase-free enzyme.
(FR)L'invention concerne un article de culture de cellules qui comprend un substrat possédant un revêtement polymère qui est favorable au passage de colonies de cellules cultivées sur le revêtement. Des exemples de revêtements polymères sont formés à partir d'acide polygalacturonique (PGA), d'alginate, ou de combinaisons de ceux-ci. Des cellules cultivées sur le revêtement polymère peuvent être séparées du substrat sous la forme d'une colonie ou d'une couche de cellules en exposant le revêtement polymère à (i) un agent de chélation, (ii) une enzyme exempte de protéinase, ou (iii) un agent de chélation et une enzyme exempte de protéinase.
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW.
African Regional Intellectual Property Organization (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG).
Publication Language: English (EN)
Filing Language: English (EN)