WIPO logo
Mobile | Deutsch | Español | Français | 日本語 | 한국어 | Português | Русский | 中文 | العربية |
PATENTSCOPE

Search International and National Patent Collections
World Intellectual Property Organization
Search
 
Browse
 
Translate
 
Options
 
News
 
Login
 
Help
 
Machine translation
1. (WO2016101782) METHOD FOR PURIFYING OXIDIZED Β-NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE
Latest bibliographic data on file with the International Bureau   

Pub. No.:    WO/2016/101782    International Application No.:    PCT/CN2015/096408
Publication Date: 30.06.2016 International Filing Date: 04.12.2015
IPC:
C07H 19/207 (2006.01), C07H 1/06 (2006.01)
Applicants: BONTAC BIO-ENGINEERING (SHENZHEN) CO., LTD [CN/CN]; Room A-2,1st Floor, Building 1 The Third Sub-Park,Taohuayuan High-Tech Innovation Park,Tiegang,Xixiang Street, Baoan District Shenzhen, Guangdong 518102 (CN)
Inventors: FU, Rongzhao; (CN).
DAI, Zhu; (CN).
ZHANG, Qi; (CN)
Priority Data:
201510255144.6 19.05.2015 CN
Title (EN) METHOD FOR PURIFYING OXIDIZED Β-NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE
(FR) PROCÉDÉ DE PURIFICATION DE PHOSPHATE DINUCLÉOTIDIQUE D'ANÉNINE B-NITOTINAMIDE OXYDÉ
(ZH) 一种氧化型β-烟酰胺腺嘌呤二核苷酸磷酸的纯化方法
Abstract: front page image
(EN)An oxidized β-nicotinamide adenine dinucleotide phosphate and a purification method therefor, specifically comprising the following steps: a. using membrane concentration equipment to perform microfiltration and then nanofiltration on a pre-treated nicotinamide adenine dinucleotide phosphate solution and obtaining a concentrated crude solution; b. adjusting the pH value of the obtained crude solution to be from 2 to 4, injecting sample and applying a preparation column for reversed-phase high performance liquid chromatography, and performing purification by gradient elution to obtain a purified sample solution; c. using the membrane concentration equipment to perform nanofiltration on the purified sample solution, using a vacuum freeze dryer to perform lyophilization, and obtaining purified nicotinamide adenine dinucleotide phosphate. The nicotinamide adenine dinucleotide phosphate prepared using the present invention has high purity, high yield, and good market prospect.
(FR)L'invention concerne un phosphate dinucléotidique d'anénine b-nitotinamide oxydé et son procédé de purification comprenant essentiellement les étapes suivantes : i. utilisation d'un équipement de concentration membranaire pour microfiltrer et nanofiltrer une solution de phosphate dinucléotidique d'anénine b-nitotinamide oxydé et obtention d'une solution concentrée; ii. réglage du pH de la solution obtenue entre 2 et 4, injection de l'échantillon et application d'une colonne de préparation pour une chromatographie liquide haute performance en phase inverse et purification par élution de gradient pour obtenir une solution d'échantillon purifiée; iii. utilisation d'un équipement de concentration membranaire sur la solution d'échantillon purifiée, utilisation d'un séchoir sous vide pour lyophiliser et obtention d'un phosphate dinucléotidique d'anénine nitotinamide oxydé. Le phosphate dinucléotidique d'anénine nitotinamide oxydée est très pur, à haut rendement et vaste application.
(ZH)本发明公开一种氧化型β-烟酰胺腺嘌呤二核苷酸磷酸及其纯化方法,其具体包括以下步骤:a、将经过预处理的辅酶Ⅱ溶液用膜浓缩设备先后进行微滤和纳滤,获得浓缩的粗品溶液;b、将获得的粗品溶液pH值调至2-4,进样上反相高效液相色谱制备柱,进行梯度洗脱纯化,得到纯化的样品溶液;c、将纯化的样品溶液用膜浓缩设备进行纳滤后,用真空冷冻干燥机冻干,获得纯化的辅酶Ⅱ。通过本发明所制备的辅酶Ⅱ纯度高,收率高,具有良好的市场前景。
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW.
African Regional Intellectual Property Organization (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG).
Publication Language: Chinese (ZH)
Filing Language: Chinese (ZH)