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1. (WO2016100128) ENHANCED PROTEIN EXPRESSION
Latest bibliographic data on file with the International Bureau

Pub. No.: WO/2016/100128 International Application No.: PCT/US2015/065296
Publication Date: 23.06.2016 International Filing Date: 11.12.2015
IPC:
C12N 15/75 (2006.01) ,C07K 14/32 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
63
Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
74
Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
75
for Bacillus
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
K
PEPTIDES
14
Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
195
from bacteria
32
from Bacillus (G)
Applicants:
DANISCO US INC [US/US]; 925 Page Mill Road Palo Alto, California 94304, US
Inventors:
BONGIORNI, Cristina; US
CHRISTENSEN, Robert, I.; US
SCHMIDT, Brian, F.; US
VAN KIMMENADE, Anita; US
Agent:
BRAZIL, Bill, T.; US
Priority Data:
62/094,75119.12.2014US
Title (EN) ENHANCED PROTEIN EXPRESSION
(FR) EXPRESSION AMÉLIORÉE DE PROTÉINE
Abstract:
(EN) The present invention relates in general to bacterial cells having a genetic alteration that results in increased expression of a protein of interest and methods of making and using such cells. Aspects of the present invention include Grampositive microorganisms, such as members of theBacillusgenus having a genetic alteration that delays, reduces, or blocks the expression or activation of genes for sporulation, thereby resulting in enhanced expression of a protein of interest. The genetic alteration is one that reduces expression of a kinA gene, a phrA gene or a phrE gene.
(FR) La présente invention concerne d'une manière générale des cellules bactériennes présentant une altération génétique qui entraîne une expression accrue d'une protéine d'intérêt, et des procédés de fabrication et d'utilisation de telles cellules. Selon des aspects, la présente invention concerne des micro-organismes Gram positif, tel que des éléments du genre Bacillus présentant une altération génétique qui retarde, réduit, ou bloque l'expression ou l'activation de gènes destinés à la sporulation, ce qui permet d'obtenir une expression améliorée d'une protéine d'intérêt. Ladite modification génétique est celle qui réduit l'expression d'un gène kinA, d'un gène phrA ou d'un gènephrE.
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Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)
Also published as:
KR1020170093247EP3234149CN107278230US20170369537JP2017538433