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1. (WO2016093606) METHOD FOR PREPARING RECOMBINANT PROTEINS THROUGH REDUCTION OF RNPA GENE EXPRESSION
Latest bibliographic data on file with the International Bureau   

Pub. No.:    WO/2016/093606    International Application No.:    PCT/KR2015/013419
Publication Date: 16.06.2016 International Filing Date: 09.12.2015
IPC:
C12N 15/63 (2006.01), C12N 9/16 (2006.01), C12P 21/00 (2006.01)
Applicants: KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY [KR/KR]; 291 Daehak-ro Yuseong-gu Daejeon 34141 (KR)
Inventors: LEE, Sang Yup; (KR).
CHUNG, Hannah; (KR)
Agent: LEE, Cheo Young; (KR)
Priority Data:
10-2014-0175780 09.12.2014 KR
10-2015-0174828 09.12.2015 KR
Title (EN) METHOD FOR PREPARING RECOMBINANT PROTEINS THROUGH REDUCTION OF RNPA GENE EXPRESSION
(FR) PROCÉDÉ DE PRÉPARATION DE PROTÉINES RECOMBINÉES PAR RÉDUCTION DE L'EXPRESSION DU GÈNE RNPA.
(KO) rnpA 유전자 발현감소를 통한 재조합단백질의 제조방법
Abstract: front page image
(EN)The present invention relates to a method for improving the production of difficult-to-express proteins in recombinant microorganisms and, more specifically, to a method for improving the production of difficult-to-express proteins using recombinant microorganisms in which sRNAs for a gene encoding a target protein and a gene encoding ribonuclease P are introduced. According to the present invention, the expression of large-sized recombinant proteins, difficult-to-express proteins, and useful proteins can be significantly increased through the reduction of rnpA gene expression.
(FR)La présente invention concerne un procédé pour améliorer la production de protéines difficiles à exprimer dans des micro-organismes recombinés et, plus spécifiquement, un procédé permettant d'améliorer la production de protéines difficiles à exprimer à l'aide de micro-organismes recombinés dans lesquels sont introduits des ARN pour un gène codant une protéine cible et un gène codant la ribonucléase P. Selon la présente invention, l'expression de protéines recombinées de grande taille, de protéines difficiles à exprimer et de protéines utiles peut être significativement augmentée grâce à la réduction de l'expression du gène rnpA.
(KO)본 발명은 재조합 미생물에서, 난발현 재조합 단백질의 생산을 향상시키는 방법에 관한 것으로, 더욱 자세하게는 목적단백질을 코딩하는 유전자와 리보뉴클레이즈 P를 코딩하는 유전자에 대한 sRNA가 되입되어 있는 재조합 미생물을 이용하여, 난발현 재조합 단백질의 생산을 향상시키는 방법에 관한 것이다. 본 발명에 따르면, rnpA 유전자의 발현 감소를 통해 큰 크기의 재조합 단백질, 난발현 단백질 및 유용 단백질의 발현을 획기적으로 증진시킬 수 있다.
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KN, KP, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW.
African Regional Intellectual Property Organization (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG).
Publication Language: Korean (KO)
Filing Language: Korean (KO)