Processing

Please wait...

Settings

Settings

1. WO2016069929 - BIOSYNTHESIS OF PRODUCTS FROM 1-CARBON COMPOUNDS

Publication Number WO/2016/069929
Publication Date 06.05.2016
International Application No. PCT/US2015/058121
International Filing Date 29.10.2015
IPC
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
P
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
7
Preparation of oxygen-containing organic compounds
24
containing a carbonyl group
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
P
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
11
Preparation of sulfur-containing organic compounds
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9
Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
88
Lyases (4.)
C12P 7/24 (2006.01)
C12P 11/00 (2006.01)
C12N 9/88 (2006.01)
CPC
C12N 15/52
C12N 15/70
C12N 9/88
C12P 1/04
C12P 11/00
C12P 13/001
Applicants
  • WILLIAM MARSH RICE UNIVERSITY [US/US]; 6100 Main Street Houston, TX 77005, US
Inventors
  • GONZALEZ, Ramon; US
  • CHOU, Alexander; US
  • CLOMBURG, James; US
Agents
  • VALOIR, Tamsen; US
Priority Data
62/069,85029.10.2014US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) BIOSYNTHESIS OF PRODUCTS FROM 1-CARBON COMPOUNDS
(FR) BIOSYNTHÈSE DE PRODUITS À PARTIR DE COMPOSÉS À UN SEUL ATOME DE CARBONE
Abstract
(EN)
An engineered microbe that contains a designed platform for the conversion of one-carbon substrates to chemical products is described. The designed platform embodies a new metabolic architecture that consolidates carbon fixation, central metabolism, and product synthesis into a single pathway. This is made possible by the key finding that 2-hydroxyacyl-CoA lyase, an enzyme in the α-oxidation pathway, is capable of catalyzing the C-C bond formation between formyl-CoA and aldehydes of different chain lengths, allowing for the elongation of the carbon backbone of said aldehyde by one-carbon units. These novel microbes present an opportunity for the production of chemicals from single-carbon feedstocks such as carbon dioxide, carbon monoxide, formate, formaldehyde, methanol or methane.
(FR)
L'invention concerne un micro-organisme génétiquement modifié qui contient une plate-forme conçue pour la conversion de substrats à un seul atome de carbone en produits chimiques. La plate-forme ainsi conçue incarne une nouvelle architecture métabolique qui regroupe la fixation du carbone, le métabolisme central et la synthèse de produits en une seule voie. Ceci est rendu possible par la découverte clé selon laquelle la 2-hydroxyacyl-CoA lyase, une enzyme de la voie d'oxydation α, est capable de catalyser la formation d'une liaison C-C entre le formyl-CoA et des aldéhydes de différentes longueurs de chaînes, ce qui permet l'allongement de la chaîne principale carbonée dudit aldéhyde par des motifs à base d'un seul atome de carbone. Ces nouveaux micro-organismes constituent une opportunité pour la production de produits chimiques à partir de matières premières à un seul atome de carbone, telles que le dioxyde de carbone, le monoxyde de carbone, le formate, le formaldéhyde, le méthanol ou le méthane.
Also published as
Latest bibliographic data on file with the International Bureau