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1. WO2016069124 - IMPROVED SINGLE MOLECULE PEPTIDE SEQUENCING

Publication Number WO/2016/069124
Publication Date 06.05.2016
International Application No. PCT/US2015/050099
International Filing Date 15.09.2015
IPC
G01N 33/68 2006.1
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
68involving proteins, peptides or amino acids
CPC
C07K 17/08
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
17Carrier-bound or immobilised peptides
02Peptides being immobilised on, or in, an organic carrier
08the carrier being a synthetic polymer
G01N 2570/00
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
2570Omics, e.g. proteomics, glycomics or lipidomics; Methods of analysis focusing on the entire complement of classes of biological molecules or subsets thereof, i.e. focusing on proteomes, glycomes or lipidomes
G01N 33/58
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
58involving labelled substances
G01N 33/582
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
58involving labelled substances
582with fluorescent label
G01N 33/6818
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
68involving proteins, peptides or amino acids
6803General methods of protein analysis not limited to specific proteins or families of proteins
6818Sequencing of polypeptides
G01N 33/6824
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
68involving proteins, peptides or amino acids
6803General methods of protein analysis not limited to specific proteins or families of proteins
6818Sequencing of polypeptides
6824involving N-terminal degradation, e.g. Edman degradation
Applicants
  • BOARD OF REGENTS, THE UNIVERSITY OF TEXAS SYSTEM [US]/[US]
Inventors
  • MARCOTTE, Edward
  • ANSLYN, Eric, V.
  • ELLINGTON, Andrew
  • SWAMINATHAN, Jagannath
  • HERNANDEZ, Erik
  • JOHNSON, Amber
  • BOULGAKOV, Alexander
  • BACHMAN, James, L.
  • SEIFERT, Helen
Agents
  • CARROLL, Peter, G.
Priority Data
62/050,46215.09.2014US
Publication Language English (en)
Filing Language English (EN)
Designated States
Title
(EN) IMPROVED SINGLE MOLECULE PEPTIDE SEQUENCING
(FR) SÉQUENÇAGE AMÉLIORÉ DES PEPTIDES D'UNE SEULE MOLÉCULE
Abstract
(EN) The present invention relates to the field of identifying proteins and peptides, and more specifically large-scale sequencing of single peptides in a mixture of diverse peptides at the single molecule level. The present invention also relates to methods for identifying amino acids in peptides, including peptides comprising unnatural amino acids. In one embodiment, the present invention contemplates labeling the N-terminal amino acid with a first label and labeling an internal amino acid with a second label. In some embodiments, the labels are fluorescent labels. In other embodiments, the internal amino acid is Lysine. In other embodiments, amino acids in peptides are identified based on the fluorescent signature for each peptide at the single molecule level.
(FR) Cette invention concerne le domaine d'identification des protéines et des peptides, et plus spécifiquement le séquençage à grande échelle des peptides individuels dans un mélange de divers peptides au niveau d'une seule molécule. L'invention concerne également des procédés d'identification d'acides aminés dans des peptides, y compris des peptides comprenant des acides aminés non naturels. Dans un mode de réalisation, l'invention comprend le marquage de l'acide aminé N-terminal avec un premier marqueur et le marquage d'un acide aminé interne avec un second marqueur. Dans certains modes de réalisation, les marqueurs sont des marqueurs fluorescents. Dans d'autres, l'acide aminé interne est Lysine. Dans d'autres modes de réalisation, les acides aminés dans les peptides sont identifiés sur la base de la signature fluorescente de chaque peptide au niveau d'une seule molécule.
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