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Pub. No.: WO/2016/065295 International Application No.: PCT/US2015/057179
Publication Date: 28.04.2016 International Filing Date: 23.10.2015
IPC:
B01D 15/34 (2006.01)
B PERFORMING OPERATIONS; TRANSPORTING
01
PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
D
SEPARATION
15
Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
08
Selective adsorption, e.g. chromatography
26
characterised by the separation mechanism
34
Size-selective separation, e.g. size-exclusion chromatography; Gel filtration; Permeation
Applicants: ABBOTT MOLECULAR INC.[US/US]; 1300 E. Touhy Avenue Des Plaines, Illinois 60018, US
Inventors: KIM, Dae Hyun; US
GUNDLING, Gerard; US
MARBLE, Herbert A.; US
ESHOO, Mark W.; US
Agent: ISENBARGER, Thomas A.; US
Priority Data:
62/068,44324.10.2014US
Title (EN) ENRICHMENT OF SMALL NUCLEIC ACIDS
(FR) ENRICHISSEMENT DE PETITS ACIDES NUCLÉIQUES
Abstract:
(EN) Provided herein is technology related to processing samples of nucleic acids and particularly, but not exclusively, to methods for enriching samples for small nucleic acids, such as small circulating cell-free DNA. Apoptotic fetal trophoblasts shed cffDNA directly into maternal blood in the placenta during gestation. It is estimated that cffDNAs are liberated into maternal plasma at a rate of approximately 20,000 per minute in 2.5 liters of maternal plasma (approximate total blood volume of a typical female is 5 liters) and are detected by some tests in circulating maternal plasma by approximately the 10th or 11th week of gestation and, in some studies, as early as the 5th week (see, e.g., Holmberg et al (2013), PLoS One 8(8):e73068) or, by some tests, as early as the 18th day of gestation (see, e.g., Guibert et al (2003) Hum Reprod 18:1733-6). A quasi-steady state relationship exists between cffDNA biogenesis in maternal plasma and cffDNA degradation by maternal plasma nucleases.
(FR) La présente invention concerne une technologie liée au traitement d'échantillons d'acides nucléiques et, en particulier, mais pas exclusivement, des procédés pour enrichir des échantillons pour des petits acides nucléiques, tels que des petits ADN exempts de cellules circulantes. Les trophoblastes foetaux apoptotiques diffusent l'ADNccf directement dans le sang maternel dans le placenta lors de la gestation. Il est estimé que les ADNcff sont libérés dans le plasma maternel à un taux d'approximativement 20000 par minute dans 2,5 litres de plasma maternel (le volume sanguin total approximatif d'une femelle type est de 5 litres) et sont détectés au moyen de certains tests dans le plasma maternel circulant approximativement à la 10ème ou 11ème semaine de gestation et, dans certaines études, dès la 5ème semaine (voir, par ex., Holmberg et al (2013), PLoS One 8(8):e73068) ou, au moyen de certains tests, dès le 18ème jour de gestation (voir, par ex., Guibert et al (2003) Hum Reprod 18:1733-6). Une relation d'état quasi-stable existe entre la biogenèse d'ADNcff dans le plasma maternel et la dégradation d'ADNcff par les nucléases du plasma maternel.
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Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)