Search International and National Patent Collections

1. (WO2016059473) SEQUENCE CONVERSION AND SIGNAL AMPLIFIER DNA HAVING LOCKED NUCLEIC ACIDS AND DETECTION METHODS USING SAME

Pub. No.:    WO/2016/059473    International Application No.:    PCT/IB2015/002141
Publication Date: Fri Apr 22 01:59:59 CEST 2016 International Filing Date: Wed Oct 14 01:59:59 CEST 2015
IPC: C12Q 1/68
C12N 15/00
Applicants: ABBOTT JAPAN CO., LTD
TOKYO INSTITUTE OF TECHNOLOGY
Inventors: KOMIYA, Ken
KOMORI, Makoto
TORU, Yoshimura
Title: SEQUENCE CONVERSION AND SIGNAL AMPLIFIER DNA HAVING LOCKED NUCLEIC ACIDS AND DETECTION METHODS USING SAME
Abstract:
Disclosed are methods for detecting a target nucleic acid in a sample. The methods include contacting said sample, in the presence of a polymerase and an endonuclease, with a sequence conversion oligonucleotide having locked nucleic acids at select positions sufficient to decrease non-specific background signal amplification. Also disclosed are methods for detecting a target nucleic acid in a sample in which said sample is contacted, in the presence of a polymerase and an endonuclease, with a sequence conversion oligonucleotide and a signal amplifier oligonucleotide, both having locked nucleic acids at select positions sufficient to decrease non-specific background signal amplification. The disclosure also provides compositions and kits comprising such sequence conversion and signal amplifier oligonucleotides.