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1. (WO2016058127) METHOD FOR BREAKING NUCLEIC ACID AND ADDING ADAPTOR BY MEANS OF TRANSPOSASE, AND REAGENT

Pub. No.:    WO/2016/058127    International Application No.:    PCT/CN2014/088542
Publication Date: Fri Apr 22 01:59:59 CEST 2016 International Filing Date: Wed Oct 15 01:59:59 CEST 2014
IPC: C12N 15/10
C12N 15/11
Applicants: BGI SHENZHEN CO., LIMITED
深圳华大基因科技有限公司
Inventors: GENG, Chunyu
耿春雨
CHEN, Ruoying
陈若莹
GUO, Rongrong
郭荣荣
ALEXEEV, Andrei
阿莱克谢耶夫安德烈
ZHANG, Yingxin
张迎新
JIANG, Hui
蒋慧
ZHANG, Wenwei
章文蔚
Title: METHOD FOR BREAKING NUCLEIC ACID AND ADDING ADAPTOR BY MEANS OF TRANSPOSASE, AND REAGENT
Abstract:
Provided are a method for breaking a nucleic acid and adding an adaptor by means of a transposase, and a reagent. The method comprises the following steps: conducting random breaking of a nucleic acid by using a transposase-embedded complex, wherein the transposase-embedded complex comprises a transposase and a first adaptor comprising a transposase identification sequence, and two ends of the broken nucleic acid are separately connected to the first adaptor and are separately provided with a gap; by means of purification or chemical reagent treatment, eliminating the influence of the transposase in the system on a follow-up reaction; connecting to a second adaptor at the gap by using a ligase, wherein a sequence of the second adaptor is different from a sequence of the first adaptor; and conducting a PCR reaction by using primers targeted to and combined with the first adaptor and the second adaptor respectively, so as to obtain a product whose two ends are respectively connected to different adaptor sequences.