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1. (WO2016056228) CAR EXPRESSION VECTOR AND CAR-EXPRESSING T CELLS
Latest bibliographic data on file with the International Bureau   

Pub. No.: WO/2016/056228 International Application No.: PCT/JP2015/005080
Publication Date: 14.04.2016 International Filing Date: 06.10.2015
Chapter 2 Demand Filed: 04.04.2016
IPC:
C12N 15/00 (2006.01) ,A61K 35/26 (2015.01) ,A61K 35/76 (2015.01) ,A61P 35/00 (2006.01) ,C12N 5/10 (2006.01) ,C12N 15/09 (2006.01) ,C07K 14/47 (2006.01) ,C07K 14/54 (2006.01) ,C07K 19/00 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
A HUMAN NECESSITIES
61
MEDICAL OR VETERINARY SCIENCE; HYGIENE
K
PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
35
Medicinal preparations containing material or reaction products thereof with undetermined constitution
12
Materials from mammals or birds
26
Lymph; Lymph-glands; Thymus
A HUMAN NECESSITIES
61
MEDICAL OR VETERINARY SCIENCE; HYGIENE
K
PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
35
Medicinal preparations containing material or reaction products thereof with undetermined constitution
66
Materials from micro-organisms
76
Viruses
A HUMAN NECESSITIES
61
MEDICAL OR VETERINARY SCIENCE; HYGIENE
P
SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
35
Antineoplastic agents
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5
Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
10
Cells modified by introduction of foreign genetic material, e.g. virus-transformed cells
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
K
PEPTIDES
14
Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
435
from animals; from humans
46
from vertebrates
47
from mammals
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
K
PEPTIDES
14
Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
435
from animals; from humans
52
Cytokines; Lymphokines; Interferons
54
Interleukins (IL)
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
K
PEPTIDES
19
Hybrid peptides
Applicants:
国立大学法人山口大学 YAMAGUCHI UNIVERSITY [JP/JP]; 山口県山口市吉田1677-1 1677-1 Yoshida, Yamaguchi-shi, Yamaguchi 7538511, JP
Inventors:
玉田 耕治 TAMADA, Koji; JP
佐古田 幸美 SAKODA, Yukimi; JP
安達 圭志 ADACHI, Keishi; JP
Agent:
廣田 雅紀 HIROTA, Masanori; JP
Priority Data:
2014-20820009.10.2014JP
Title (EN) CAR EXPRESSION VECTOR AND CAR-EXPRESSING T CELLS
(FR) VECTEUR D'EXPRESSION DE RÉCEPTEUR D'ANTIGÈNE CHIMÉRIQUE, ET CELLULE T D'EXPRESSION DE RÉCEPTEUR D'ANTIGÈNE CHIMÉRIQUE
(JA) CAR発現ベクター及びCAR発現T細胞
Abstract:
(EN) The present invention addresses the problem of providing chimeric antigen receptor (CAR)-expressing T cells that express a CAR and also express a T cell immunological function promoting factor, and that have a strong immunity-inducing effect and high antitumor activity. The present invention also addresses the problem of providing a CAR expression vector for producing such CAR-expressing T cells. This CAR expression vector contains a nucleic acid that codes for a CAR, and a nucleic acid that codes for a T cell immunological function promoting factor, wherein the nucleic acid that codes for the T cell immunological function promoting factor comprises either: the nucleic acid that codes for interleukin-7 and the nucleic acid that codes for CCL19; a nucleic acid that codes for a dominant-negative mutant of SHP-1; or a nucleic acid that codes for a dominant-negative mutant of SHP-2. CAR-expressing T cells into which said CAR expression vector has been introduced are produced.
(FR) L'invention a pour objet de fournir : une cellule T d'expression de récepteur d'antigène chimérique qui exprime un promoteur de fonction immunitaire de cellule T avec un récepteur d'antigène chimérique (CAR) dans une cellule T, et qui présente un effet induisant une immunité et une activité antitumorale qui sont élevées; et un vecteur d'expression de récepteur d'antigène chimérique destiné à produire cette cellule T d'expression de récepteur d'antigène chimérique. Le vecteur d'expression de récepteur d'antigène chimérique comprend un acide nucléique codant le récepteur d'antigène chimérique, et un acide nucléique codant le promoteur de fonction immunitaire de la cellule T. L'acide nucléique codant ledit promoteur de fonction immunitaire, consiste en un acide nucléique codant une interleukine 7 ainsi qu'un acide nucléique codant un CCL19, en un acide nucléique codant un mutant dominant négatif vis-à-vis d'un SHP-1, ou un acide nucléique codant mutant dominant négatif vis-à-vis d'un SHP-2. Ainsi, l'invention permet de produire un vecteur d'expression de récepteur d'antigène chimérique, et une cellule T d'expression de récepteur d'antigène chimérique dans laquelle ledit vecteur d'expression de récepteur d'antigène chimérique est induit.
(JA)  T細胞においてキメラ抗原受容体(CAR)と共にT細胞の免疫機能促進因子を発現し、免疫誘導効果や抗腫瘍活性が高いCAR発現T細胞や、かかるCAR発現T細胞を作製するためのCAR発現ベクターを提供することを課題とする。 キメラ抗原受容体(CAR)をコードする核酸及びT細胞の免疫機能促進因子をコードする核酸を含有するCAR発現ベクターであって、前記免疫機能促進因子をコードする核酸が、インターロイキン7をコードする核酸及びCCL19をコードする核酸、SHP-1に対するドミナントネガティブ変異体をコードする核酸、又はSHP-2に対するドミナントネガティブ変異体をコードする核酸であるCAR発現ベクターや、前記CAR発現ベクターを導入したCAR発現T細胞を作製する。
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: Japanese (JA)
Filing Language: Japanese (JA)