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1. (WO2016055169) AUTOMATIC METHOD FOR MONITORING CELL CULTURE GROWTH

Pub. No.:    WO/2016/055169    International Application No.:    PCT/EP2015/060297
Publication Date: Fri Apr 15 01:59:59 CEST 2016 International Filing Date: Tue May 12 01:59:59 CEST 2015
IPC: C12M 1/34
C12M 1/36
G01N 35/00
Applicants: FRAUNHOFER-GESELLSCHAFT ZUR FÖRDERUNG DER ANGEWANDTEN FORSCHUNG E.V.
Inventors: DRÖGE, Marc
MÜLLER, Christian
LINNEMANN, Anja
MATHIS, Harald Peter
BORBE, Stefan
HEUP, Torsten
PIPPOW, Andreas
Title: AUTOMATIC METHOD FOR MONITORING CELL CULTURE GROWTH
Abstract:
The invention relates to an automated method for monitoring cell culture growth, in particular bacterial growth, wherein a receiving dish (24) is provided with a nutrient medium (38), on and/or in which a cell culture is applied, in particular a sample of human or animal tissue, such as blood, to which bacteria has been added, and which also contains one or a number of reagents, in particular antibiotics. A microscope (10) having a camera (18) and an image-capturing plane that can move along the optical axis (20) is provided, and the receiving dish (24) is brought into the microscope (10) in the nutrient medium (38), in order to monitor a potential growth of the cell culture. An image of the nutrient medium (38) is captured using the camera (18) of the microscope (10), at pre-determinable time intervals in the in particular single-digit minute region.