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1. (WO2016052769) METHOD FOR MANUFACTURING MICRO-HEMISPHERE ARRAY PLATE, MICROFLUIDIC DEVICE COMPRISING MICRO-HEMISPHERE ARRAY PLATE, AND METHOD FOR CULTURING CELL AGGREGATE USING SAME

Pub. No.:    WO/2016/052769    International Application No.:    PCT/KR2014/009070
Publication Date: Fri Apr 08 01:59:59 CEST 2016 International Filing Date: Tue Sep 30 01:59:59 CEST 2014
IPC: B29C 33/38
G01N 33/50
C12M 3/00
Applicants: MICROFIT CO., LTD.
(주) 마이크로핏
Inventors: OH, Hyun-Jik
오현직
NO, Da-Yoon
노다윤
LEE, Sang-Hoon
이상훈
Title: METHOD FOR MANUFACTURING MICRO-HEMISPHERE ARRAY PLATE, MICROFLUIDIC DEVICE COMPRISING MICRO-HEMISPHERE ARRAY PLATE, AND METHOD FOR CULTURING CELL AGGREGATE USING SAME
Abstract:
The present invention relates to a method for manufacturing a micro-hemisphere array plate, a microfluidic device comprising the micro-hemisphere array plate, and a method for culturing a cell aggregate using the same. It is possible to form a cell aggregate having excellent condition if cells are cultured in three dimensions by using the method for manufacturing a micro-hemisphere array plate, the microfluidic device comprising the micro-hemisphere array plate, and the method for culturing a cell aggregate using the same according to the present invention. In particular, because cells are cultured in a condition more similar to an environment within the human body, it is possible to form a cell aggregate having a better condition than by existing cell-culturing methods. The cell aggregate cultured in the present invention can be directly used in cell therapy, and it is possible to culture and then obtain cells that are difficult to obtain artificially. Moreover, because cells are cultured under a condition similar to the human body, it is possible to form a cell aggregate of cells that are difficult to agglomerate in three dimensions. Also, it is possible to co-culture two or more kinds of cells, and in such a case, it is possible to form a cell aggregate of excellent quality because cells are cultured under a condition similar to the human body. Eventually, according to the present invention, it is possible to achieve a groundbreaking development in drug screening or cytotoxicity and in various tests.