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1. (WO2016052769) METHOD FOR MANUFACTURING MICRO-HEMISPHERE ARRAY PLATE, MICROFLUIDIC DEVICE COMPRISING MICRO-HEMISPHERE ARRAY PLATE, AND METHOD FOR CULTURING CELL AGGREGATE USING SAME
Latest bibliographic data on file with the International Bureau   

Pub. No.:    WO/2016/052769    International Application No.:    PCT/KR2014/009070
Publication Date: 07.04.2016 International Filing Date: 29.09.2014
IPC:
B29C 33/38 (2006.01), G01N 33/50 (2006.01), C12M 3/00 (2006.01)
Applicants: MICROFIT CO., LTD. [KR/KR]; #306 Jinrikwan, 161, Jeongneung-ro Seongbuk-gu Seoul 136-703 (KR)
Inventors: OH, Hyun-Jik; (KR).
NO, Da-Yoon; (KR).
LEE, Sang-Hoon; (KR)
Agent: CHUNG HYUN PATENT & LAW FIRM; Hanmaum Bldg. 4F., 225, Baumoe-ro Seocho-gu Seoul 137-130 (KR)
Priority Data:
Title (EN) METHOD FOR MANUFACTURING MICRO-HEMISPHERE ARRAY PLATE, MICROFLUIDIC DEVICE COMPRISING MICRO-HEMISPHERE ARRAY PLATE, AND METHOD FOR CULTURING CELL AGGREGATE USING SAME
(FR) PROCÉDÉ PERMETTANT LA FABRICATION D’UNE PLAQUE PORTANT UN RÉSEAU DE MICRO-HÉMISPHÈRES, DISPOSITIF MICROFLUIDIQUE COMPRENANT LA PLAQUE PORTANT UN RÉSEAU DE MICRO-HÉMISPHÈRES ET PROCÉDÉ PERMETTANT LA CULTURE D’AGRÉGAT CELLULAIRE UTILISANT CE DERNIER
(KO) 마이크로 반구체 어레이 플레이트의 제조방법, 마이크로 반구체 어레이 플레이트를 포함하는 미세유체소자 및 이를 이용한 세포 집합체의 배양방법
Abstract: front page image
(EN)The present invention relates to a method for manufacturing a micro-hemisphere array plate, a microfluidic device comprising the micro-hemisphere array plate, and a method for culturing a cell aggregate using the same. It is possible to form a cell aggregate having excellent condition if cells are cultured in three dimensions by using the method for manufacturing a micro-hemisphere array plate, the microfluidic device comprising the micro-hemisphere array plate, and the method for culturing a cell aggregate using the same according to the present invention. In particular, because cells are cultured in a condition more similar to an environment within the human body, it is possible to form a cell aggregate having a better condition than by existing cell-culturing methods. The cell aggregate cultured in the present invention can be directly used in cell therapy, and it is possible to culture and then obtain cells that are difficult to obtain artificially. Moreover, because cells are cultured under a condition similar to the human body, it is possible to form a cell aggregate of cells that are difficult to agglomerate in three dimensions. Also, it is possible to co-culture two or more kinds of cells, and in such a case, it is possible to form a cell aggregate of excellent quality because cells are cultured under a condition similar to the human body. Eventually, according to the present invention, it is possible to achieve a groundbreaking development in drug screening or cytotoxicity and in various tests.
(FR)La présente invention se rapporte à un procédé permettant la fabrication d’une plaque portant un réseau de micro-hémisphères, à un dispositif microfluidique comprenant la plaque portant un réseau de micro-hémisphères et à un procédé pour la culture d’un agrégat cellulaire utilisant ce dernier. Il est possible de former un agrégat cellulaire ayant un excellent état si des cellules sont cultivées en trois dimensions à l’aide du procédé permettant la fabrication d’une plaque portant un réseau de micro-hémisphères, du dispositif microfluidique comprenant la plaque portant un réseau de micro-hémisphères et du procédé pour la culture d’un agrégat cellulaire utilisant ce dernier selon la présente invention. En particulier, du fait que des cellules sont cultivées dans des conditions plus similaires à celles d’un environnement au sein du corps humain, il est possible de former un agrégat cellulaire ayant un meilleur état que par des procédés de culture cellulaire existants. L’agrégat cellulaire cultivé dans la présente invention peut être directement utilisé en thérapie cellulaire et il est possible de cultiver et ensuite d’obtenir des cellules qui sont difficiles à obtenir artificiellement. De plus, du fait que des cellules sont cultivées dans des conditions similaires à celles du corps humain, il est possible de former un agrégat cellulaire constitué de cellules qui sont difficiles à agglomérer en trois dimensions. De plus, il est possible de co-cultiver deux ou plus de deux sortes de cellules et, dans un tel cas, il est possible de former un agrégat cellulaire d’excellente qualité parce que des cellules sont cultivées dans des conditions similaires à celles du corps humain. Enfin, selon la présente invention, il est possible de réaliser un développement novateur en criblage de médicament ou cytotoxicité et dans divers essais.
(KO)본 발명은 마이크로 반구체어레이 플레이트의 제조방법, 마이크로 반구체 어레이 플레이트를 포함하는 미세유체소자 및 이를 이용한 세포 집합체의 배양방법에 관한 것이다. 본 발명에 따른 마이크로 반구체 어레이 플레이트의 제조방법, 마이크로 반구체 어레이 플레이트를 포함하는 미세유체소자 및 이를 이용한 세포 집합체의 배양방법을 이용하여 세포를 3차원적으로 배양하게 되면 상태가 우수한 세포 집합체의 형성이 가능하다. 특히 인체 내의 환경에 보다 유사한 조건으로 세포를 배양하기 때문에 기존에 존재하던 세포 배양 방법들보다 우수한 상태로 세포 집합체를 형성하는 것이 가능하다. 이러한 본 발명을 통해 배양된 세포 집합체는 세포 치료에 직접적으로 쓰일 수 있으며, 인공적으로는 수득하기 어려운 세포를 배양하여 얻는 것이 가능하다. 또한 인체와 유사한 조건을 제공하여 배양하기 때문에 3차원적으로는 뭉쳐지기 어려운 세포의 세포 집합체 형성이 가능하다. 또한 두 가지 이상의 세포를 공동 배양하는 것이 가능하고, 이때에도 인체와 유사한 조건으로 배양하기 때문에 우수한 품질의 세포 집합체 형성이 가능하다. 결구 이러한 본 발명을 통해 약물 스크리닝이나 세포 독성, 그리고 각종의 테스트에 획기적 발전을 달성 할 수 있다.
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW.
African Regional Intellectual Property Organization (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG).
Publication Language: Korean (KO)
Filing Language: Korean (KO)