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1. (WO2016052657) METHOD FOR CULTURING PLURIPOTENT STEM CELLS
Latest bibliographic data on file with the International Bureau   

Pub. No.: WO/2016/052657 International Application No.: PCT/JP2015/077824
Publication Date: 07.04.2016 International Filing Date: 30.09.2015
Chapter 2 Demand Filed: 29.07.2016
IPC:
C12N 5/10 (2006.01) ,C12N 5/0735 (2010.01) ,C12N 5/074 (2010.01)
Applicants: NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY[JP/JP]; 3-1, Kasumigaseki 1-chome, Chiyoda-ku, Tokyo 1008921, JP (JP)
JTEC CORPORATION[JP/JP]; 4-35, Saito Yamabuki 2-chome, Ibaraki-shi, Osaka 5670086, JP
Inventors: UEMURA Toshimasa; JP
ONOMURA Yui; JP
TSUMURA Takashi; JP
Agent: HIRAKI Yusuke; JP
Priority Data:
2014-20187530.09.2014JP
2015-01767930.01.2015JP
Title (EN) METHOD FOR CULTURING PLURIPOTENT STEM CELLS
(FR) PROCÉDÉ DE CULTURE DE CELLULES SOUCHES PLURIPOTENTES
(JA) 万能性幹細胞の培養方法
Abstract: front page image
(EN) Provided is a method for efficiently culturing pluripotent stem cells at a higher safety. The present invention relates to: a method for culturing pluripotent stem cells, said method comprising culturing isolated pluripotent stem cells in a quasi-microgravity environment, thus proliferating the pluripotent stem cells while retaining the pluripotent stem cells in undifferentiated conditions, and thereby forming and developing spheroids of the pluripotent stem cells; and a method for inducing differentiation of pluripotent stem cells using the aforesaid method.
(FR) Cette invention concerne un procédé de culture efficace de cellules souches pluripotentes à un niveau de sécurité plus élevé. Un procédé de culture de cellules souches pluripotentes, ledit procédé comprenant la culture de cellules souches pluripotentes isolées dans un environnement de quasi microgravité, pour faire ainsi proliférer lesdites cellules souches pluripotentes tout en les maintenant à l'état non différencié, et former ainsi et faire se développer des sphéroïdes de cellules souches pluripotentes est décrit ; ainsi qu'un procédé d'induction de différenciation desdites cellules souches pluripotentes à l'aide du procédé précité.
(JA) 万能性幹細胞のより安全性が高く効率的な培養方法が提供される。本発明は、単離された万能性幹細胞を擬微小重力環境下で培養することにより、万能性幹細胞を未分化性を保持した状態で増殖させ、万能性幹細胞のスフェロイドを形成及び成長させることを含む、万能性幹細胞の培養方法、及びその方法を用いた万能性幹細胞の分化誘導方法に関する。
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: Japanese (JA)
Filing Language: Japanese (JA)