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1. (WO2016052472) METHOD FOR PRODUCING THREE-DIMENSIONAL CELL AGGREGATES

Pub. No.:    WO/2016/052472    International Application No.:    PCT/JP2015/077447
Publication Date: Fri Apr 08 01:59:59 CEST 2016 International Filing Date: Wed Sep 30 01:59:59 CEST 2015
IPC: C12N 5/07
C12M 3/00
Applicants: PUBLIC UNIVERSITY CORPORATION YOKOHAMA CITY UNIVERSITY
公立大学法人横浜市立大学
OSAKA UNIVERSITY
国立大学法人大阪大学
NATIONAL UNIVERSITY CORPORATION NAGOYA UNIVERSITY
国立大学法人名古屋大学
Inventors: YOKOYAMA, Utako
横山 詩子
ISHIKAWA, Yoshihiro
石川 義弘
KANEKO, Makoto
金子 真
SAKUMA, Shinya
佐久間 臣耶
ARAI, Fumihito
新井 史人
Title: METHOD FOR PRODUCING THREE-DIMENSIONAL CELL AGGREGATES
Abstract:
Disclosed is a means for producing thick cell aggregates by a simple process without requiring a procedure of detaching cells and then overlaying the cells on each other. A method for producing three-dimensional cell aggregates according to the present invention comprises: a cell accommodation step of accommodating a cell suspension in a cell accommodation vessel; and a pressure application step of applying a pressure to cells in the vessel. Each of the cell accommodation step and the pressure application step may be repeated several times. According to the method of the present invention, it becomes possible to produce thick and highly tough cell aggregates by a simple procedure of applying a pressure to a cell suspension or a cell-containing medium. In the method, the damage to the cells is extremely small and the cells can be maintained in a good condition, because a procedure of overlaying multiple cell sheets on each other is not necessary, either. Therefore, the cell aggregates are advantageous for use as a tissue graft.