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1. (WO2016051517) LUCIFERASE HAVING EMISSION OF ORANGE LIGHT
Latest bibliographic data on file with the International Bureau   

Pub. No.: WO/2016/051517 International Application No.: PCT/JP2014/076113
Publication Date: 07.04.2016 International Filing Date: 30.09.2014
IPC:
C12N 15/09 (2006.01) ,C12N 9/02 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9
Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
02
Oxidoreductases (1.), e.g. luciferase
Applicants: OLYMPUS CORPORATION[JP/JP]; 43-2, Hatagaya 2-chome, Shibuya-ku, Tokyo 1510072, JP
Inventors: OGO, Katsunori; JP
Agent: KURATA, Masatoshi; JP
Priority Data:
Title (EN) LUCIFERASE HAVING EMISSION OF ORANGE LIGHT
(FR) LUCIFÉRASE ÉMETTANT DE LA LUMIÈRE ORANGE
(JA) オレンジ色の発光を示すルシフェラーゼ
Abstract:
(EN) A luciferase according to an embodiment comprises an amino acid sequence produced by introducing a mutation into the amino acid sequence represented by SEQ ID NO: 38. The luciferase according to the embodiment can catalyze a luminescent reaction through which light having a maximum emission wavelength ranging from 570 to 610 nm can be emitted. The emitted light has an intensity 10 times greater than that of light emitted through a luminescent reaction catalyzed with a luciferase comprising the amino acid sequence represented by SEQ ID NO: 48.
(FR) L'invention porte, selon un mode de réalisation, sur une luciférase comprenant une séquence d'acides aminés produite par introduction d'une mutation dans la séquence d'acides aminés représentée par SEQ ID N° : 38. La luciférase selon le mode de réalisation peut catalyser une réaction luminescente grâce à laquelle de la lumière ayant une longueur d'onde d'émission maximale allant de 570 à 610 nm peut être émise. La lumière émise a une intensité 10 fois plus grande que celle de lumière émise grâce à une réaction luminescente catalysée par une luciférase comprenant la séquence d'acides aminés représentée par SEQ ID N° : 48.
(JA)  実施形態に係るルシフェラーゼは、配列番号38のアミノ酸配列に対して変異が導入されたアミノ酸配列を有する。実施形態に係るルシフェラーゼは、570nmから610nmの最大発光波長を有する発光を生じる発光反応を触媒する。前記発光は、配列番号48のアミノ酸配列を有するルシフェラーゼに触媒される発光反応の発光と比較して、10倍以上の強度を有する。
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Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, KE, KG, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: Japanese (JA)
Filing Language: Japanese (JA)