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1. (WO2016049986) METHOD FOR SEPARATING UMBILICAL CORD MESENCHYMAL STEM CELLS
Latest bibliographic data on file with the International Bureau   

Pub. No.:    WO/2016/049986    International Application No.:    PCT/CN2014/094957
Publication Date: 07.04.2016 International Filing Date: 25.12.2014
IPC:
C12N 5/0775 (2010.01)
Applicants: SICHUAN NEO-LIFE STEM CELL BIOTECH INC. [CN/CN]; 15 Jin Quan Road, Jin Niu District Chengdu, Sichuan 610000 (CN)
Inventors: ZHONG, Liwu; (CN).
LAI, Zhenyang; (CN).
FAN, Zhaoxin; (CN).
WU, Mingjun; (CN).
ZHANG, Bo; (CN).
CHEN, Qiang; (CN)
Agent: GAOYUNG INTELLECTUAL PROPERTY AGENCY(GENERALPARTNERSHIP); The Yiguanmiao Post Office Box A-42 (Ph.D.Entrepreneur Park,No.5 Gaopeng Road), High-Tech District Chengdu, Sichuan 610041 (CN)
Priority Data:
201410515345.0 29.09.2014 CN
Title (EN) METHOD FOR SEPARATING UMBILICAL CORD MESENCHYMAL STEM CELLS
(FR) PROCÉDÉ DE SÉPARATION DE CELLULES SOUCHES MÉSENCHYMATEUSES DE CORDON OMBILICAL
(ZH) 一种脐带间充质干细胞的分离方法
Abstract: front page image
(EN)Provided is a method for separating umbilical cord mesenchymal cells, comprising the following steps: (1) taking a fresh umbilical cord, cutting short and removing the blood stains; (2) cutting into tissue blocks with a volume of 2-3 mm3, evenly plating on a culture dish coated with an extracellular matrix, and culturing under the conditions of 37ºC and 5% of CO2 for 1-4 h, then adding a culture medium for the mesenchymal stem cells and continuing the culturing under the conditions of 37ºC and 5% of CO2, and (3) on the fifth day of culturing, half changing the culture medium; on the eighth day of culturing, removing all the tissue blocks and totally changing the culture medium; and harvesting cells at a cell confluence of 30-40%.
(FR)L'invention concerne un procédé de séparation de cellules mésenchymateuses de cordon ombilical, comprenant les étapes suivantes, consistant à : (1) prendre un cordon ombilical frais, le découper et enlever les taches de sang; (2) découper en blocs de tissu d'un volume de 2-3 mm3, les étaler uniformément sur un récipient de culture revêtu d'une matrice extracellulaire et cultiver dans des conditions de 37°C et 5 % de CO2 pendant 1-4 h puis ajouter un milieu de culture pour les cellules souches mésenchymateuses et continuer la culture dans des conditions de 37°C et 5 % de CO2 et (3) le cinquième jour de culture, changer la moitié du milieu de culture; le huitième jour de culture, retirer tous les blocs de tissu et changer complètement le milieu de culture; et récolter les cellules à une confluence cellulaire de 30-40 %.
(ZH)提供了一种脐带间充质干细胞的分离方法,包括如下步骤:(1)取新鲜脐带,剪短,去除血污;(2)剪碎成体积为2~3mm3的组织块,均匀平铺于包被了细胞外基质的培养皿中,在37℃、5%CO 2条件下培养1~4h,然后添加间充质干细胞培养基,在37℃、5%CO 2条件下继续培养;(3)在培养第5天时,对培养基进行半换液;在培养第8天时,去掉所有组织块,并对培养基进行全换液;在细胞融合度为30-40%时收获细胞。
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW.
African Regional Intellectual Property Organization (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG).
Publication Language: Chinese (ZH)
Filing Language: Chinese (ZH)